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Literature summary for 1.1.1.B20 extracted from

  • Guo, Z.; Zhao, X.; He, Y.; Yang, T.; Gao, H.; Li, G.; Chen, F.; Sun, M.; Lee, J.K.; Zhang, L.
    Efficient (3R)-acetoin production from meso-2,3-butanediol using a new whole-cell biocatalyst with co-expression of meso-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin (2016), J. Microbiol. Biotechnol., 27, 92-100 .
    View publication on PubMed
Search for more literature for 1.1.1.B20

Cloned(Commentary)

Cloned (Comment) Organism
gene mbdh, functional coexpression of Serratia sp. meso-2,3-butanediol dehydrogenase, Lactobacillus brevis NADH oxidase and Vitreoscilla sp. hemoglobin in Escherichia coli strain BL21(DE3)/pET-mbdh-nox-vgb, construction of plasmids pET-mbdh, pET-mbdh-nox, and pET-mbdh-nox-vgb, method optimization Serratia sp.
gene mbdh, recombinant expression of meso-2,3-BDH in Escherichia coli strain BL21(DE3), coexpression with NADH oxidase (NOX) from Lactobacillus brevis, and hemoglobin protein (VHB) from Vitreoscilla, subcloning in Escherichia coli strain DH5alpha Serratia sp. T241

Protein Variants

Protein Variants Comment Organism
additional information efficient (3R)-acetoin production from meso-2,3-butanediol using a whole-cell biocatalyst with coexpression of Serratia sp. meso-2,3-butanediol dehydrogenase, Lactobacillus brevis NADH oxidase and Vitreoscilla sp. hemoglobin Serratia sp.
additional information chiral (3R)-AC production from meso-2,3-butanediol (meso-2,3-BD) is obtained using recombinant Escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from Serratia sp. T241, Lactobacillus brevis, and Vitreoscilla, respectively. The biocatalysis system of Escherichia coli/pET-mbdh-nox-vgb is developed and the bioconversion conditions are optimized. Under the optimal conditions, 86.74 g/l of (3R)-acetoin with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% is achieved from 93.73 g/l meso-2,3-BD using the whole-cell biocatalysis system, pH 7.0 at 30°C for 12 h. The results show the industrial potential for (3R)-acetoin production via whole-cell biocatalysis. Escherichia coli/pET-mbdh cannot produce acetoin from (2R,3R)-2,3-BD as substrate. To obtain high (3R)-acetoin productivity, a cofactor regeneration system involved in co-expression of meso-2,3-BDH and NOX enzymes from Serratia sp. T241 Lactobacillus brevis is developed in Escherichia coli. The NOX enzyme efficiently oxidizes NADH, which is formed by meso-2,3-BDH, and regenerate NAD+ for the biocatalytic process. The feasibility of (3R)-AC production from the substrate of meso-2,3-BD by whole-cell biocatalysis is conducted, method optimization, overview. A small amount of (3S)-acetoin (1.86 g/l) can also be produced from (2S,3S)-2,3-BD in the substrate 2,3-BD (2.23% of (2S,3S)-2,3-BD) by the biocatalyst Serratia sp. T241

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates Serratia sp. T241
Mg2+ activates Serratia sp. T241
additional information poor effects by Mn2+, Fe3+, and Ca2+ Serratia sp. T241

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-butane-2,3-diol + NAD+ Serratia sp.
-
 (3R)-acetoin + NADH + H+
-
 r
(2R,3S)-butane-2,3-diol + NAD+ Serratia sp. T241
-
 (3R)-acetoin + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Serratia sp. AEF50077
-
-
Serratia sp. T241
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.17
-
 crude extracet of Escherichia coli cells expressing the enzyme from plasmid pET-mbdh-nox-vgb, pH 8.0, 22°C Serratia sp.
2.47
-
 crude extracet of Escherichia coli cells expressing the enzyme from plasmid pET-mbdh-nox, pH 8.0, 22°C Serratia sp.
3.32
-
 crude extracet of Escherichia coli cells expressing the enzyme from plasmid pET-mbdh, pH 8.0, 22°C Serratia sp.
3.32
-
 recombinant enzyme, pH 8.0, 22°C Serratia sp. T241

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-butane-2,3-diol + NAD+
-
 Serratia sp. (3R)-acetoin + NADH + H+
-
 r
(2R,3S)-butane-2,3-diol + NAD+
-
 Serratia sp. T241 (3R)-acetoin + NADH + H+
-
 ?
additional information meso-2,3-BDH from Serratia sp. T241 exhibits higher catalytic efficiency compared with the meso-2,3-BDHs from Klebsiella pneumoniae strain XJ-Li and Serratia marcescens strain H30. No activity is detected for (2R,3R)-2,3-BD as substrate by meso-2,3-BDH, but meso-2,3-BDH from Serratia sp. T241 can efficiently convert (2S,3S)-2,3-BD and meso-2,3-BD into (3S)-acetoin and (3R)-acetoin, respectively, cf. EC 1.1.1.76 Serratia sp. T241 ?
-
-

Synonyms

Synonyms Comment Organism
mbdh
-
 Serratia sp.
mbdh
-
 Serratia sp. T241
meso-2,3-BDH
-
 Serratia sp.
meso-2,3-BDH
-
 Serratia sp. T241
meso-2,3-butanediol dehydrogenase
-
 Serratia sp.
More cf. EC 1.1.1.76 Serratia sp. T241

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Serratia sp. T241
22
-
 in vitro assay at room temperature Serratia sp.
30 37 in vivo assay at Serratia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in vivo assay at Serratia sp.
8
-
 assay at Serratia sp. T241
8
-
 in vitro assay at Serratia sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Serratia sp.
NAD+
-
 Serratia sp. T241
NADH
-
 Serratia sp.