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Literature summary for 1.1.1.95 extracted from
- D-3-Phosphoglycerate dehydrogenase (2018), Front. Mol. Biosci., 5, 110 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphooxypyruvate | significant substrate inhibition | Homo sapiens |  |
| 3-phosphooxypyruvate | significant substrate inhibition. NADH bound at or near the ASB site and reduces the amount of substrate inhibition due to substrate interaction at the ASB site | Mycobacterium tuberculosis | |
| L-serine | potent inhibitor | Escherichia coli | |
| additional information | CBR5884, a potent inhibitor of the human enzyme (PGDH), does not inhibit the enzyme from Mycobacterium tuberculosis | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
3-phosphooxypyruvate | pH and temperature not specified in the publication | Escherichia coli | |
| 0.015 | - |
3-phosphooxypyruvate | pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.025 | - |
NADH | pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.027 | - |
NAD+ | pH 7.0, temperature not specified in the publication | Rattus norvegicus | |
| 0.088 | - |
2-oxoglutarate | pH and temperature not specified in the publication | Escherichia coli | |
| 0.17 | - |
3-phosphooxypyruvate | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.4 | - |
D-2-hydroxyglutarate | pH and temperature not specified in the publication | Escherichia coli | |
| 0.54 | - |
3-phospho-D-glycerate | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
| 1.2 | - |
3-phospho-D-glycerate | pH and temperature not specified in the publication | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glycerate + NAD+ | Homo sapiens | - |
3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | Mycobacterium tuberculosis | enzyme in the L-serine biosynthetic pathway | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | Escherichia coli | enzyme in the L-serine biosynthetic pathway | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | Rattus norvegicus | enzyme in the L-serine biosynthetic pathway | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | Mycobacterium tuberculosis ATCC 25618 | enzyme in the L-serine biosynthetic pathway | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | C3SVM7 | - |
- |
| Homo sapiens | O43175 | - |
- |
| Mycobacterium tuberculosis | P9WNX3 | - |
- |
| Mycobacterium tuberculosis ATCC 25618 | P9WNX3 | - |
- |
| Rattus norvegicus | O08651 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NADH + H+ | - |
Escherichia coli | D-2-hydroxyglutarate + NAD+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | - |
Homo sapiens | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | - |
Homo sapiens | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | - |
Mycobacterium tuberculosis | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | - |
Escherichia coli | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | - |
Rattus norvegicus | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | enzyme in the L-serine biosynthetic pathway | Mycobacterium tuberculosis | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | enzyme in the L-serine biosynthetic pathway | Escherichia coli | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | enzyme in the L-serine biosynthetic pathway | Rattus norvegicus | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | - |
Mycobacterium tuberculosis ATCC 25618 | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | enzyme in the L-serine biosynthetic pathway | Mycobacterium tuberculosis ATCC 25618 | 3-phosphooxypyruvate + NADH + H+ | - |
? | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Homo sapiens | 3-phospho-D-glycerate + NAD+ | - |
r | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Mycobacterium tuberculosis | 3-phospho-D-glycerate + NAD+ | - |
r | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Escherichia coli | 3-phospho-D-glycerate + NAD+ | - |
r | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Rattus norvegicus | 3-phospho-D-glycerate + NAD+ | - |
r | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Mycobacterium tuberculosis ATCC 25618 | 3-phospho-D-glycerate + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-3-phosphoglycerate dehydrogenase | - |
Homo sapiens |
| D-3-phosphoglycerate dehydrogenase | - |
Mycobacterium tuberculosis |
| D-3-phosphoglycerate dehydrogenase | - |
Escherichia coli |
| D-3-phosphoglycerate dehydrogenase | - |
Rattus norvegicus |
| PGDH | - |
Homo sapiens |
| PGDH | - |
Mycobacterium tuberculosis |
| PGDH | - |
Escherichia coli |
| PGDH | - |
Rattus norvegicus |
| Rv2996c | - |
Mycobacterium tuberculosis |
| serA1 | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
3-phospho-D-glycerate | pH and temperature not specified in the publication | Escherichia coli | |
| 0.7 | - |
D-2-hydroxyglutarate | pH and temperature not specified in the publication | Escherichia coli | |
| 1.4 | - |
3-phospho-D-glycerate | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
| 2 | 8 | 3-phosphooxypyruvate | pH and temperature not specified in the publication | Escherichia coli | |
| 12 | - |
2-oxoglutarate | pH and temperature not specified in the publication, 12-33/s | Escherichia coli | |
| 2400 | - |
3-phosphooxypyruvate | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
| NAD+ | - |
Mycobacterium tuberculosis | |
| NAD+ | - |
Escherichia coli | |
| NAD+ | - |
Rattus norvegicus | |
| NADH | - |
Homo sapiens | |
| NADH | - |
Escherichia coli | |
| NADH | - |
Rattus norvegicus | |
| NADH | NADH can bind to the enzyme in the absence of substrate but that the binding constants were too slow to account for the catalytic reaction | Mycobacterium tuberculosis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| drug target | potential cancer therapy target | Homo sapiens |
| malfunction | PGDH deficiency results in metabolic defects of the nervous system whose systems range from microcephaly at birth, seizures, and psychomotor retardation | Homo sapiens |
| metabolism | enzyme in the L-serine biosynthetic pathway | Mycobacterium tuberculosis |
| metabolism | enzyme in the L-serine biosynthetic pathway | Escherichia coli |
| metabolism | enzyme in the L-serine biosynthetic pathway | Rattus norvegicus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
3-phospho-D-glycerate | pH and temperature not specified in the publication | Escherichia coli | |
| 2 | - |
D-2-hydroxyglutarate | pH and temperature not specified in the publication | Escherichia coli | |
| 400 | - |
2-oxoglutarate | pH and temperature not specified in the publication | Escherichia coli | |
| 9000 | - |
3-phosphooxypyruvate | pH and temperature not specified in the publication | Escherichia coli | |
| 10000 | - |
3-phosphooxypyruvate | pH and temperature not specified in the publication | Mycobacterium tuberculosis | |
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