Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-phosphohydroxypyruvate | substrate inhibition | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
phosphohydroxypyruvate | pH 6.5 | Entamoeba histolytica | |
0.0177 | - |
NADH | pH 6.5 | Entamoeba histolytica | |
0.0867 | - |
NAD+ | pH 9.0 | Entamoeba histolytica | |
0.141 | - |
NADPH | pH 6.5 | Entamoeba histolytica | |
0.212 | - |
3-phosphoglycerate | pH 9.0 | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal salt concentration is 350-400 mM | Entamoeba histolytica | |
NaCl | optimal salt concentration is 350-400 mM | Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
2 * 33500, calculation from nucleotide sequence | Entamoeba histolytica |
70000 | 74000 | gel filtration | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q76KF5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Entamoeba histolytica |
Storage Stability | Organism |
---|---|
-80°C, 50% glycerol, purified recombinant enzyme remains active after 1 month of storage | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NADP+ | - |
Entamoeba histolytica | 3-phosphohydroxypyruvate + NADPH + H+ | - |
? | |
3-phosphoglycerate + NAD+ | - |
Entamoeba histolytica | 3-phosphohydroxypyruvate + NADH | - |
r | |
additional information | no activity with 2-oxoglutarate | Entamoeba histolytica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33500, calculation from nucleotide sequence | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
D-phosphoglycerate dehydrogenase | - |
Entamoeba histolytica |
EhPGDH | - |
Entamoeba histolytica |
PGDH | - |
Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | reaction with 3-phosphohydroxypyruvate + NADH | Entamoeba histolytica |
9 | - |
reaction with 3-phosphoglycerate and NAD+ | Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Entamoeba histolytica | |
NADPH | less efficiently than NADH | Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | calculation from nucleotide sequence | - |
6.9 |