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Literature summary for 1.1.1.95 extracted from

  • Ali, V.; Hashimoto, T.; Shigeta, Y.; Nozaki, T.
    Molecular and biochemical characterization of D-phosphoglycerate dehydrogenase from Entamoeba histolytica. A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways (2004), Eur. J. Biochem., 271, 2670-2681.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
3-phosphohydroxypyruvate substrate inhibition Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
phosphohydroxypyruvate pH 6.5 Entamoeba histolytica
0.0177
-
NADH pH 6.5 Entamoeba histolytica
0.0867
-
NAD+ pH 9.0 Entamoeba histolytica
0.141
-
NADPH pH 6.5 Entamoeba histolytica
0.212
-
3-phosphoglycerate pH 9.0 Entamoeba histolytica

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal salt concentration is 350-400 mM Entamoeba histolytica
NaCl optimal salt concentration is 350-400 mM Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
2 * 33500, calculation from nucleotide sequence Entamoeba histolytica
70000 74000 gel filtration Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica Q76KF5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Entamoeba histolytica

Storage Stability

Storage Stability Organism
-80°C, 50% glycerol, purified recombinant enzyme remains active after 1 month of storage Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glycerate + NADP+
-
Entamoeba histolytica 3-phosphohydroxypyruvate + NADPH + H+
-
?
3-phosphoglycerate + NAD+
-
Entamoeba histolytica 3-phosphohydroxypyruvate + NADH
-
r
additional information no activity with 2-oxoglutarate Entamoeba histolytica ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33500, calculation from nucleotide sequence Entamoeba histolytica

Synonyms

Synonyms Comment Organism
D-phosphoglycerate dehydrogenase
-
Entamoeba histolytica
EhPGDH
-
Entamoeba histolytica
PGDH
-
Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 reaction with 3-phosphohydroxypyruvate + NADH Entamoeba histolytica
9
-
reaction with 3-phosphoglycerate and NAD+ Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
NADH
-
Entamoeba histolytica
NADPH less efficiently than NADH Entamoeba histolytica

pI Value

Organism Comment pI Value Maximum pI Value
Entamoeba histolytica calculation from nucleotide sequence
-
6.9