Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rickettsia prowazekii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | heterologous expression in Escherichia coli complements an Escherichia coli gpsA mutant | Rickettsia prowazekii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
NADPH | pH 7.4, temperature not specified in the publication | Rickettsia prowazekii | |
0.528 | - |
glycerone phosphate | cosubstrate NADPH, pH 7.4, temperature not specified in the publication | Rickettsia prowazekii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36600 | - |
x * 36600, SDS-PAGE and calculated for His-tagged protein | Rickettsia prowazekii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rickettsia prowazekii | Q9ZDA0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Rickettsia prowazekii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate + NADH + H+ | - |
Rickettsia prowazekii | sn-glycerol 3-phosphate + NAD+ | - |
r | |
glycerone phosphate + NADPH + H+ | favoured reaction direction | Rickettsia prowazekii | sn-glycerol 3-phosphate + NADP+ | - |
r | |
sn-glycerol 3-phosphate + NADP+ | - |
Rickettsia prowazekii | glycerone phosphate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 36600, SDS-PAGE and calculated for His-tagged protein | Rickettsia prowazekii |
Synonyms | Comment | Organism |
---|---|---|
G3PDH | - |
Rickettsia prowazekii |
GpsA | - |
Rickettsia prowazekii |
RP442 | - |
Rickettsia prowazekii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is preferred over NADH as the cofactor | Rickettsia prowazekii | |
NADPH | NADPH is preferred over NADH as the cofactor | Rickettsia prowazekii |
General Information | Comment | Organism |
---|---|---|
physiological function | Rickettsia prowazekii is unable to synthesize glycerone phosphate as a substrate for the GpsA enzymatic reaction. Purified Rickettsia prowazekii transports and incorporates glycerone phosphate into phospholipids, implicating a role for GpsA in vivo as part of a rickettsial G3P acquisition pathway for phospholipid biosynthesis | Rickettsia prowazekii |