Protein Variants | Comment | Organism |
---|---|---|
additional information | existence of a naturally occurring feedback inhibition-resistant mutant in strain BB26-36-R2 | Escherichia coli K-12 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sn-glycerol-3-phosphate | the wild-type enzyme is feedback inhibited | Escherichia coli K-12 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25900 | - |
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
26000 | - |
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
32500 | - |
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
48300 | - |
feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
49000 | - |
wild-type enzyme, gel filtration | Escherichia coli K-12 |
50000 | - |
feedback inhibition-resistant mutant, gel filtration | Escherichia coli K-12 |
50700 | - |
wild-type enzyme, sedimentation equilibrium analysis | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate + NAD(P)H | Escherichia coli K-12 | the enzyme is required for biosynthesis of sn-glycerol-3-phosphate, overview | sn-glycerol-3-phosphate + NAD(P)+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | P0A6S7 | and strain BB26-36-R2 | - |
Purification (Comment) | Organism |
---|---|
native wild-type enzyme from Escherichia coli K12 12000fold by streptomycin and ammonum sulfate fractionation, adsorption, hydroxy apatite, and anion exchange chromatography, and gel filtration to over 95% purity, feedback inhibition-resistant mutant from strain BB26-36-R2 | Escherichia coli K-12 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70 | - |
purified wild-type enzyme | Escherichia coli K-12 |
Storage Stability | Organism |
---|---|
-20°C, purified concentrated wild-type enzyme, 50% glycerol, 25 mM Tris-HCl, pH 7.4, 0.5 mM DTT, 0.2 M NaCl, 5 mM glycerol-3-phosphate, 1 month, over 90% remaining activity | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate + NAD(P)H | i.e. dihydroxyacetone phosphate | Escherichia coli K-12 | sn-glycerol-3-phosphate + NAD(P)+ | - |
r | |
glycerone phosphate + NAD(P)H | the enzyme is required for biosynthesis of sn-glycerol-3-phosphate, overview | Escherichia coli K-12 | sn-glycerol-3-phosphate + NAD(P)+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
Synonyms | Comment | Organism |
---|---|---|
sn-glycerol-3-phosphate dehydrogenase | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli K-12 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli K-12 | |
NADH | - |
Escherichia coli K-12 | |
NADP+ | - |
Escherichia coli K-12 | |
NADPH | - |
Escherichia coli K-12 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli K-12 | isoelectric focusing | - |
6 |