Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tartronate | inhibition of oxidative decarboxylation of D-malate | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
(2R,3R)-3-iodomalate | - |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
enzyme expressed in Escherichia coli carrying the plasmid pTDH1, which expresses the gene encoding TDH at high levels | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-3-iodomalate + NAD+ | - |
Pseudomonas putida | 3-iodopyruvate + NADH + CO2 | - |
? | |
D-malate + NAD+ | - |
Pseudomonas putida | pyruvate + CO2 + NADH | - |
? | |
L-tartrate + NAD+ | - |
Pseudomonas putida | oxaloglycolate + NADH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | D-malate oxidation is largely limited by the rate of decarboxylation of the intermediate oxaloacetate which occurs at 660 per min. Hydride transfer from D-malate to NAD+ occurs with a rate constant of 1800 per min | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Pseudomonas putida |