Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | Taiwanofungus camphoratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
veratraldehyde | at pH 6.0 temperature not specified in the publication | Taiwanofungus camphoratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
1 * 39000, SDS-PAGE | Taiwanofungus camphoratus |
40580 | - |
- |
Taiwanofungus camphoratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taiwanofungus camphoratus | V9HZX4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Taiwanofungus camphoratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Taiwanofungus camphoratus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dimethoxybenzyl alcohol + NADP+ | - |
Taiwanofungus camphoratus | 2,4-dimethoxybenzyl aldehyde + NADPH + H+ | - |
? | |
3,4-dimethoxybenzyl alcohol + NADP+ | - |
Taiwanofungus camphoratus | ? | - |
? | |
3,4-dimethoxybenzyl alcohol + NADP+ | - |
Taiwanofungus camphoratus | veratraldehyde + NADPH + H+ | - |
r | |
4-(hydroxymethyl)benzoic acid + NAD+ | - |
Taiwanofungus camphoratus | ? + NADH + H+ | - |
? | |
benzaldehyde + NADPH + H+ | reduction of benzaldehyde at pH 6.0 is NADPH-dependent while NADH does not yield detectable activity under the same conditions | Taiwanofungus camphoratus | benzyl alcohol + NADP+ | - |
r | |
benzyl alcohol + NAD+ | oxidation of benzyl alcohol at pH 9.6 is NAD+-dependent while NADP+ did not yield detectable activity under the same conditions | Taiwanofungus camphoratus | benzaldehyde + NADPH + H+ | - |
r | |
benzyl alcohol + NADP+ | - |
Taiwanofungus camphoratus | benzaldehyde + NADPH + H+ | - |
? | |
veratraldehyde + NADPH + H+ | - |
Taiwanofungus camphoratus | 3,4-dimethoxybenzyl alcohol + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, SDS-PAGE | Taiwanofungus camphoratus |
monomer | 1 * 40580, MALDI-TOF mass spectrometry | Taiwanofungus camphoratus |
Synonyms | Comment | Organism |
---|---|---|
AAD | - |
Taiwanofungus camphoratus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
the enzyme's half-life of deactivation at 58°C is 4.2 min. There is no detectable enzyme activity when it is heated above 60°C | Taiwanofungus camphoratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
with veratraldehyde as substrate | Taiwanofungus camphoratus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | at pH 8.0 and 10.0 the enzyme retains 62% activity, while the enzyme retains 13% activity at pH 4.0 | Taiwanofungus camphoratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | oxidation of benzyl alcohol at pH 9.6 is NAD+-dependent | Taiwanofungus camphoratus | |
NADP+ | - |
Taiwanofungus camphoratus | |
NADPH | reduction of benzaldehyde at pH 6.0 is NADPH-dependent | Taiwanofungus camphoratus |