Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Yokenella sp. WZY002 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Yokenella sp. WZY002 | |
Cu2+ | - |
Yokenella sp. WZY002 | |
Zn2+ | - |
Yokenella sp. WZY002 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on | Yokenella sp. WZY002 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36411 | - |
- |
Yokenella sp. WZY002 |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide | Yokenella sp. WZY002 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yokenella sp. WZY002 | W6CX26 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + NADPH + H+ | highest reductive activity with benzaldehyde | Yokenella sp. WZY002 | benzyl alcohol + NADP+ | - |
r | |
benzyl alcohol + NADP+ | - |
Yokenella sp. WZY002 | benzaldehyde + NADPH + H+ | - |
r | |
crotonaldehyde + NADPH + H+ | - |
Yokenella sp. WZY002 | crotonyl alcohol + NADP+ | - |
r | |
crotonyl alcohol + NADP+ | highest oxidative activity with crotyl alcohol | Yokenella sp. WZY002 | crotonaldehyde + NADPH + H+ | - |
r | |
additional information | no activity with NADH or NAD+ | Yokenella sp. WZY002 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36411, calculated from amino acid sequence | Yokenella sp. WZY002 |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Yokenella sp. WZY002 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
for crotonyl alcohol oxidation | Yokenella sp. WZY002 |
65 | - |
for crotonaldehyde reduction | Yokenella sp. WZY002 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
the enzyme shows moderate thermostability with a half-life of 6.2 h at 55°C | Yokenella sp. WZY002 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
for crotonaldehyde reduction | Yokenella sp. WZY002 |
8 | - |
for crotonyl alcohol oxidation | Yokenella sp. WZY002 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Yokenella sp. WZY002 | |
NADPH | - |
Yokenella sp. WZY002 |