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Literature summary for 1.1.1.9 extracted from

  • Guo, J.; Huang, S.; Chen, Y.; Guo, X.; Xiao, D.
    Heterologous expression of Spathaspora passalidarum xylose reductase and xylitol dehydrogenase genes improved xylose fermentation ability of Aureobasidium pullulans (2018), Microb. Cell Fact., 17, 64 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.9

Cloned(Commentary)

Cloned (Comment) Organism
gene XYL2.1, recombinant expression in Aureobasidium pullulans strain CBS 110374 Spathaspora passalidarum

Protein Variants

Protein Variants Comment Organism
additional information the combinatorial expression of two xylose reductase (XR) genes and two xylitol dehydrogenase (XDH) genes from Spathaspora passalidarum and the heterologous expression of the Piromyces sp. xylose isomerase (XI) gene are induced in Aureobasidium pullulans strain CBS 110374. Overexpression of XYL1.2 (encoding XR) and XYL2.2 (encoding XDH) is the most beneficial for xylose utilization, resulting in a 17.76% increase in consumed xylose compared with the parent strain, whereas the introduction of the Piromyces sp. XI pathway fails to enhance xylose utilization efficiency. Construction of knock-in mutants, method, evaluation of internal redox state, xylose utilization and cell growth of the recombinant strains, pH 5.8, 28°C, comparison the fermentation abilities of the parent strain and the recombinant strains using several carbon sources, overview Spathaspora passalidarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Spathaspora passalidarum
-
 D-xylulose + NADH + H+
-
 r
xylitol + NAD+ Spathaspora passalidarum 11-Y1
-
 D-xylulose + NADH + H+
-
 r
xylitol + NAD+ Spathaspora passalidarum NRRL Y-27907
-
 D-xylulose + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Spathaspora passalidarum G3AIB3 gene XYL2.1
-
Spathaspora passalidarum G3AIP8 gene XYL2.2
-
Spathaspora passalidarum 11-Y1 G3AIB3 gene XYL2.1
-
Spathaspora passalidarum 11-Y1 G3AIP8 gene XYL2.2
-
Spathaspora passalidarum NRRL Y-27907 G3AIP8 gene XYL2.2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
 Spathaspora passalidarum D-xylulose + NADH + H+
-
 r
xylitol + NAD+
-
 Spathaspora passalidarum 11-Y1 D-xylulose + NADH + H+
-
 r
xylitol + NAD+
-
 Spathaspora passalidarum NRRL Y-27907 D-xylulose + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
XDH
-
 Spathaspora passalidarum
XYL2.1
-
 Spathaspora passalidarum
XYL2.2
-
 Spathaspora passalidarum
xylitol dehydrogenase
-
 Spathaspora passalidarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 in vivo assay at, recombinant enzyme Spathaspora passalidarum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Spathaspora passalidarum
NADH
-
 Spathaspora passalidarum