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Literature summary for 1.1.1.9 extracted from

  • Tani, T.; Taguchi, H.; Akamatsu, T.
    Analysis of metabolisms and transports of xylitol using xylose- and xylitol-assimilating Saccharomyces cerevisiae (2017), J. Biosci. Bioeng., 123, 613-620 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene XYL2, recombinant expression in Saccharomyces cerevisiae strain SCB7 resulting in strains SCF202 and SCF201, coexpression of XYL2 with XYL1, encoding a xylose reductase, and the endogenous XKS1, encoding a xylulokinase, all under the control of the TDH3 promoter in the chromosomal DNA, the recombinant strain efficiently grows in minimal medium containing xylose as the sole carbon source. An additional mutations of MTH1, encoding the endogenous negative regulator of the glucose-sensing signal transduction pathway, MTH1e32, and GRR1, encoding endogenous Grr1, an Fbox protein component of an SCF ubiquitineligase complex grr1e33, enable efficient growth in medium containing high xylose concentrations, subcloning in Escherichia coli strain DH10B Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Scheffersomyces stipitis
-
D-xylulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis
-
formerly Pichia stipitis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
Scheffersomyces stipitis D-xylulose + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
NAD+-dependent XDH
-
Scheffersomyces stipitis
XDH
-
Scheffersomyces stipitis
XYL2
-
Scheffersomyces stipitis
xylitol dehydrogenase
-
Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Scheffersomyces stipitis

General Information

General Information Comment Organism
metabolism the cofactor imbalance between the NAD(P)H-dependent wild type XR and NAD+-dependent XDH can create an intracellular redox imbalance, leading to an accumulation of NADH and a shortage of NAD+ necessary for the XDH reaction. The likely increase in intracellular xylitol concentration favors xylitol excretion, which reduces the ethanol yield by Saccharomyces cerevisiae Scheffersomyces stipitis