Cloned (Comment) | Organism |
---|---|
gene xdhA | Aspergillus oryzae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of deletion mutants of xdhA by homologous transformation, to generate mutants with decreased dehydrogenase activities and increased xylitol production. Gene pyrG is used as a selectable marker. Consumption of D-xylose for xdhA2-1 and ladA2-1 is similar to that of KBN616. Mutant xdhA2-1 displays the highest xylitol productivity. XdhA-disrupted mutant xdhA2-1 exhibits the highest xylitol productivity from D-xylose and is selected for further production of xylitol from oat spelt xylan | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | Aspergillus oryzae | - |
D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | Aspergillus oryzae P5 | - |
D-xylulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q86ZV0 | a DELTApyrG strain developed from strain KBN616 | - |
Aspergillus oryzae P5 | Q86ZV0 | a DELTApyrG strain developed from strain KBN616 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | - |
Aspergillus oryzae | D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Aspergillus oryzae P5 | D-xylulose + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-xylulose reductase A | - |
Aspergillus oryzae |
xdhA | - |
Aspergillus oryzae |
xylitol dehydrogenase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aspergillus oryzae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Aspergillus oryzae |
General Information | Comment | Organism |
---|---|---|
additional information | xylitol production of wild-type and mutant strains, overview | Aspergillus oryzae |