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Literature summary for 1.1.1.9 extracted from

  • Mahmud, A.; Hattori, K.; Hongwen, C.; Kitamoto, N.; Suzuki, T.; Nakamura, K.; Takamizawa, K.
    Xylitol production by NAD+-dependent xylitol dehydrogenase (xdhA)- and L-arabitol-4-dehydrogenase (ladA)-disrupted mutants of Aspergillus oryzae (2013), J. Biosci. Bioeng., 115, 353-359.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xdhA Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutants of xdhA by homologous transformation, to generate mutants with decreased dehydrogenase activities and increased xylitol production. Gene pyrG is used as a selectable marker. Consumption of D-xylose for xdhA2-1 and ladA2-1 is similar to that of KBN616. Mutant xdhA2-1 displays the highest xylitol productivity. XdhA-disrupted mutant xdhA2-1 exhibits the highest xylitol productivity from D-xylose and is selected for further production of xylitol from oat spelt xylan Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Aspergillus oryzae
-
D-xylulose + NADH + H+
-
?
xylitol + NAD+ Aspergillus oryzae P5
-
D-xylulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q86ZV0 a DELTApyrG strain developed from strain KBN616
-
Aspergillus oryzae P5 Q86ZV0 a DELTApyrG strain developed from strain KBN616
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
Aspergillus oryzae D-xylulose + NADH + H+
-
?
xylitol + NAD+
-
Aspergillus oryzae P5 D-xylulose + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
D-xylulose reductase A
-
Aspergillus oryzae
xdhA
-
Aspergillus oryzae
xylitol dehydrogenase
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Aspergillus oryzae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Aspergillus oryzae

General Information

General Information Comment Organism
additional information xylitol production of wild-type and mutant strains, overview Aspergillus oryzae