Literature summary for 1.1.1.9 extracted from

  • Tiwari, M.K.; Moon, H.J.; Jeya, M.; Lee, J.K.
    Cloning and characterization of a thermostable xylitol dehydrogenase from Rhizobium etli CFN42 (2010), Appl. Microbiol. Biotechnol., 87, 571-581.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhizobium etli

Crystallization (Commentary)

Crystallization (Comment) Organism
multi-template homology modeling. The structural model of XDH obtained consists of a classical alpha/beta Rossmann fold pattern commonly found in the MDR family, which is organized into two beta-barrel domains, the coenzyme-binding residues 163-300 and catalytic residues 1-162, 301-364 Rhizobium etli

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1-10 mM, strong inhibition Rhizobium etli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
NAD+ pH 9.5, 25°C Rhizobium etli
12.1
-
xylitol pH 9.5, 25°C Rhizobium etli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ or Mn2+, required Rhizobium etli
Ca2+ 1 mM, 18% of the activity with Mg2+ Rhizobium etli
Co2+ 1 mM, 38% of the activity with Mg2+ Rhizobium etli
Cu2+ 1 mM, 51% of the activity with Mg2+ Rhizobium etli
Zn2+ 1 mM, 62% of the activity with Mg2+. The deduced XDH gene product possesses the Zn2+-containing ADH signature sequence [GHE]xx[G]xxxxx[G]xx[V] and the catalytic Zn2+-binding site Cys41, His66, Glu67, and Glu152 Rhizobium etli
additional information activity is not significantly stimulated by Ba2+, Ca2+, Fe2+, Hg2+, or K+ Rhizobium etli
Mn2+ or Mg2+, required. At 1 mM, 108% of the activity with Mg2+ Rhizobium etli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
gel filtration Rhizobium etli
34000
-
4 * 35858, calculated, 4 * 34000, SDS-PAGE Rhizobium etli
35858
-
4 * 35858, calculated, 4 * 34000, SDS-PAGE Rhizobium etli

Organism

Organism UniProt Comment Textmining
Rhizobium etli Q2K0Q7 strain CFN42
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzmye Rhizobium etli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
Rhizobium etli D-xylulose + NADH + H+
-
?
D-sorbitol + NAD+ 90% of the activity with xylitol Rhizobium etli D-fructose + NADH + H+
-
?
D-mannitol + NAD+ 8% of the activity with xylitol Rhizobium etli ? + NADH + H+
-
?
erythritol + NAD+ 0.77% of the activity with xylitol Rhizobium etli ? + NADH + H+
-
?
galactitol + NAD+ 0.98% of the activity with xylitol Rhizobium etli ? + NADH + H+
-
?
L-arabinitol + NAD+ 21% of the activity with xylitol Rhizobium etli ? + NADH + H+
-
?
L-threitol + NAD+ 0.05% of the activity with xylitol Rhizobium etli ? + NADH + H+
-
?
L-threitol + NAD+ 75% of the activity with xylitol Rhizobium etli ? + NADH + H+
-
?
ribitol + NAD+ 40% of the activity with xylitol Rhizobium etli ? + NADH + H+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 35858, calculated, 4 * 34000, SDS-PAGE Rhizobium etli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Rhizobium etli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
half-life 120 min Rhizobium etli
53.1
-
half denaturation temperature Rhizobium etli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Rhizobium etli

Cofactor

Cofactor Comment Organism Structure
additional information activity with NADP+ is 4% of the activity with NAD+ Rhizobium etli
NAD+ the deduced XDH gene product possesses an NAD-binding glycine-rich Rossmann fold domain [GXGXXG] present in MDR superfamily Rhizobium etli