Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Blastobotrys adeninivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
D-ribulose | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
0.9 | - |
L-sorbose | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
2 | - |
D-xylulose | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
4 | - |
xylitol | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
4 | - |
D-sorbitol | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
5.2 | - |
xylitol | 35°C, pH 7.5, recombinant enzyme | Blastobotrys adeninivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40300 | - |
2 * 40300, calculated from sequence | Blastobotrys adeninivorans |
72000 | - |
native enzyme, gel filtration, non-denaturing PAGE | Blastobotrys adeninivorans |
80000 | - |
recombinant enzyme, gel filtration, non-denaturing PAGE | Blastobotrys adeninivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastobotrys adeninivorans | Q6KAV2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Blastobotrys adeninivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose + NADH + H+ | - |
Blastobotrys adeninivorans | ? | - |
? | |
D-sorbitol + NAD+ | - |
Blastobotrys adeninivorans | D-fructose + NADH + H+ | - |
ir | |
L-sorbose + NADH + H+ | - |
Blastobotrys adeninivorans | ? | - |
? | |
ribitol + NAD+ | - |
Blastobotrys adeninivorans | ? | - |
? | |
xylitol + NAD+ | - |
Blastobotrys adeninivorans | D-xylulose + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40300, calculated from sequence | Blastobotrys adeninivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
native and recombinant enzyme | Blastobotrys adeninivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
74 | - |
D-ribulose | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
216 | - |
L-sorbose | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
302.9 | - |
D-xylulose | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
528 | - |
D-sorbitol | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans | |
2115 | - |
xylitol | 35°C, pH 7.5, recombinant enzyme | Blastobotrys adeninivorans | |
2644 | - |
xylitol | 35°C, pH 7.5, native enzyme | Blastobotrys adeninivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
native and recombinant enzyme | Blastobotrys adeninivorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.9 | stable | Blastobotrys adeninivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no activity with NADP+ | Blastobotrys adeninivorans | |
NADH | no activity with NADPH | Blastobotrys adeninivorans |