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Literature summary for 1.1.1.9 extracted from

  • Panagiotou, G.; Kekos, D.; Macris, B.J.; Christakopoulos, P.
    Purification and characterisation of NAD+-dependent xylitol dehydrogenase from Fusarium oxysporum. (2002), Biotechnol. Lett., 24, 2089-2092.
    View publication on PubMed

General Stability

General Stability Organism
30°C, pH 6-7, 24 h, fully stable Fusarium oxysporum

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 10 mM, 8% inhibition Fusarium oxysporum
CuSO4 10 mM, no residual activity Fusarium oxysporum
EDTA dialysis overnight, 80% residual activity Fusarium oxysporum
MgCl2 10 mM, 4% inhibition Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
NAD+
-
Fusarium oxysporum
94
-
D-xylitol
-
Fusarium oxysporum

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 10 mM, increase of activity by 100% Fusarium oxysporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
2 * 48000, SDS-PAGE Fusarium oxysporum
94000
-
PAGE Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Fusarium oxysporum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
250
-
pH 8.4, 30°C Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sorbitol + NAD+ 69% of activity against D-xylitol Fusarium oxysporum D-fructose + NADH + H+
-
?
D-xylitol + NAD+
-
Fusarium oxysporum D-xylulose + NADH + H+
-
?
additional information no substrate: glycerol Fusarium oxysporum ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Fusarium oxysporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
1 h, completely stable Fusarium oxysporum
50
-
1 h, no residual activity Fusarium oxysporum

pH Range

pH Minimum pH Maximum Comment Organism
9 10 fully active Fusarium oxysporum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 24 h, fully stable Fusarium oxysporum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Fusarium oxysporum
NADH
-
Fusarium oxysporum

pI Value

Organism Comment pI Value Maximum pI Value
Fusarium oxysporum isoelectric focusing
-
4.2