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Literature summary for 1.1.1.9 extracted from

  • Takamizawa, K.; Uchida, S.; Hatsu, M.; Suzuki, T.; Kawai, K.
    Development of a xylitol biosensor composed of xylitol dehydrogenase and diaphorase (2000), Can. J. Microbiol., 46, 350-357.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 NAD+
-
 Candida tropicalis
49.8
-
 xylitol
-
 Candida tropicalis

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
130
-
-
 Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribitol + NAD+
-
 Candida tropicalis D-ribulose + NADH
-
 ?
D-sorbitol + NAD+
-
 Candida tropicalis D-fructose + NADH + H+
-
 ?
D-xylulose + NADH + H+
-
 Candida tropicalis xylitol + NAD+
-
 r
xylitol + NAD+
-
 Candida tropicalis D-xylulose + NADH + H+
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
NAD+ xylitol oxidation favors NAD+ over NADP+, but xylulose reduction favors NADPH over NADH Candida tropicalis
NADP+ xylitol oxidation favors NAD+ over NADP+, but xylulose reduction favores NADPH over NADH Candida tropicalis