KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
NADH | - |
Scheffersomyces shehatae | |
0.16 | - |
D-sorbitol | - |
Scheffersomyces shehatae | |
13.8 | - |
xylulose | - |
Scheffersomyces shehatae | |
18.5 | - |
xylitol | - |
Scheffersomyces shehatae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Scheffersomyces shehatae | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Scheffersomyces shehatae |
82000 | - |
gel filtration | Scheffersomyces shehatae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Xylitol + NAD+ | Scheffersomyces shehatae | inducible pathway of xylose catabolism | ? | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces shehatae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Scheffersomyces shehatae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.95 | - |
- |
Scheffersomyces shehatae |
Storage Stability | Organism |
---|---|
-20°C, stable with 50% v/v glycerol | Scheffersomyces shehatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribitol + NAD+ | - |
Scheffersomyces shehatae | D-ribulose + NADH | - |
r | |
D-ribulose + NADH | - |
Scheffersomyces shehatae | D-ribitol + NAD+ | - |
r | |
D-sorbitol + NAD+ | oxidation at about 45% | Scheffersomyces shehatae | D-fructose + NADH + H+ | - |
? | |
D-xylulose + NADH + H+ | - |
Scheffersomyces shehatae | xylitol + NAD+ | - |
r | |
additional information | no oxidation of ribitol | Scheffersomyces shehatae | ? | - |
? | |
additional information | no oxidation of mannitol | Scheffersomyces shehatae | ? | - |
? | |
Xylitol + NAD+ | inducible pathway of xylose catabolism | Scheffersomyces shehatae | ? | - |
r | |
xylitol + NAD+ | - |
Scheffersomyces shehatae | D-xylulose + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Scheffersomyces shehatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
xylitol formation | Scheffersomyces shehatae |
8.6 | - |
D-xylulose formation | Scheffersomyces shehatae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Scheffersomyces shehatae | |
additional information | at high concentrations of xylitol 1.5% as effective as NAD+ | Scheffersomyces shehatae | |
additional information | very low activity with NADPH | Scheffersomyces shehatae | |
NAD+ | - |
Scheffersomyces shehatae | |
NADH | - |
Scheffersomyces shehatae |