General Stability | Organism |
---|---|
4-vinylpyridine, 20 min, 0°C, 76% activity | Pseudomonas mevalonii |
5,5'-dithiobis(2-nitrobenzoic acid), 20 min, 0°C, 20% activity | Pseudomonas mevalonii |
N-ethylmaleimide, 20 min, 0°C, 0% activity | Pseudomonas mevalonii |
p-hydroxymercuribenzoate, 20 min, 0°C, 19% activity | Pseudomonas mevalonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetate | competivive inhibitor vs. mevalonate, non inhibitory vs. NAD+ | Pseudomonas mevalonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
R-mevalonate | oxidative acylation of mevalonate | Pseudomonas mevalonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
alpha4, 4 * 43000, SDS-PAGE | Pseudomonas mevalonii |
178000 | - |
gel filtration | Pseudomonas mevalonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + NADH + H+ | Pseudomonas mevalonii | mevalonate is first commited intermediate in polyisoprenoid synthesis | (R)-mevalonate + NAD+ + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mevalonii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.5 | - |
mevalonate acylation | Pseudomonas mevalonii |
Storage Stability | Organism |
---|---|
0°C, 10 hours, 5% loss of activity | Pseudomonas mevalonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mevalonate + CoA + 2 NAD+ | - |
Pseudomonas mevalonii | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | - |
r | |
(S)-3-hydroxy-3-methylglutaryl-CoA + NADH + H+ | mevalonate is first commited intermediate in polyisoprenoid synthesis | Pseudomonas mevalonii | (R)-mevalonate + NAD+ + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 43000, SDS-PAGE | Pseudomonas mevalonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
oxidative acylation of mevalonate | Pseudomonas mevalonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
oxidative acylation of mevalonate | Pseudomonas mevalonii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas mevalonii | |
NADH | reverse reaction | Pseudomonas mevalonii |