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Literature summary for 1.1.1.86 extracted from

  • Lv, Y.; Kandale, A.; Wun, S.J.; McGeary, R.P.; Williams, S.J.; Kobe, B.; Sieber, V.; Schembri, M.A.; Schenk, G.; Guddat, L.W.
    Crystal structure of Mycobacterium tuberculosis ketol-acid reductoisomerase at 1.0 A resolution - a potential target for anti-tuberculosis drug discovery (2016), FEBS J., 283, 1184-1196.
    View publication on PubMed
Search for more literature for 1.1.1.86

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme with two magnesium ions bound in the active site, hanging drop vapor diffusion method, using 250 mM MgCl2, 20% (w/v) PEG 3350, 100 mM Tris/HCl, pH 8.0 Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
N-Hydroxy-N-isopropyloxamate
-
 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 (S)-2-acetolactate at pH 8.5 and 37°C Mycobacterium tuberculosis
2.1
-
 3-hydroxypyruvate at pH 8.5 and 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 multi-angle light scattering Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-acetolactate + NADPH + H+ Mycobacterium tuberculosis
-
 (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
(S)-2-acetolactate + NADPH + H+ Mycobacterium tuberculosis ATCC 25618
-
 (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
2-aceto-2-hydroxybutyrate + NADPH + H+ Mycobacterium tuberculosis
-
 ? + NADP+
-
 r
2-aceto-2-hydroxybutyrate + NADPH + H+ Mycobacterium tuberculosis ATCC 25618
-
 ? + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WKJ7
-
-
Mycobacterium tuberculosis ATCC 25618 P9WKJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose resin column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-acetolactate + NADPH + H+
-
 Mycobacterium tuberculosis (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
(S)-2-acetolactate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 (R)-2,3-dihydroxyisovalerate + NADP+
-
 r
2-aceto-2-hydroxybutyrate + NADPH + H+
-
 Mycobacterium tuberculosis ? + NADP+
-
 r
2-aceto-2-hydroxybutyrate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 ? + NADP+
-
 r
3-hydroxypyruvate + NADPH + H+
-
 Mycobacterium tuberculosis ? + NADP+
-
 r
3-hydroxypyruvate + NADPH + H+
-
 Mycobacterium tuberculosis ATCC 25618 ? + NADP+
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 37155, mass spectrometry Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
KARI
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 (S)-2-acetolactate at pH 8.5 and 37°C Mycobacterium tuberculosis
12.5
-
 3-hydroxypyruvate at pH 8.5 and 37°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000974
-
 N-Hydroxy-N-isopropyloxamate at pH 8.5 and 37°C Mycobacterium tuberculosis