Literature summary for 1.1.1.86 extracted from

Tadrowski, S.; Pedroso, M.M.; Sieber, V.; Larrabee, J.A.; Guddat, L.W.; Schenk, G.
Metal ions play an essential catalytic role in the mechanism of ketol-acid reductoisomerase (2016), Chemistry, 22, 7427-7436.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Escherichia coli
Mg2+	dependent on	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+	Escherichia coli	-	(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+	-	r
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+	Oryza sativa	-	(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Oryza sativa	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+	-	Escherichia coli	(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+	-	r
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+	-	Oryza sativa	(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+	-	r
2-acetolactate + NADPH + H+	-	Escherichia coli	2,3-dihydroxy-3-methylbutanoate + NADP+	-	?
2-acetolactate + NADPH + H+	-	Oryza sativa	2,3-dihydroxy-3-methylbutanoate + NADP+	-	?
3-hydroxypyruvate + NADPH + H+	-	Escherichia coli	?	-	?
3-hydroxypyruvate + NADPH + H+	-	Oryza sativa	?	-	?

Synonyms

Synonyms	Comment	Organism
KARI	-	Escherichia coli
KARI	-	Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.2	-	2-acetolactate	at pH 8.0, temperature not specified in the publication	Escherichia coli
1.4	-	2-acetolactate	at pH 8.0, temperature not specified in the publication	Oryza sativa
4.5	-	3-hydroxypyruvate	at pH 8.0, temperature not specified in the publication	Oryza sativa
26	-	3-hydroxypyruvate	at pH 8.0, temperature not specified in the publication	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	9	-	Escherichia coli
8	-	-	Oryza sativa

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Escherichia coli
NADP+	-	Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.4	-	2-acetolactate	at pH 8.0, temperature not specified in the publication	Escherichia coli
3.3	-	3-hydroxypyruvate	at pH 8.0, temperature not specified in the publication	Escherichia coli
4.16	-	3-hydroxypyruvate	at pH 8.0, temperature not specified in the publication	Oryza sativa
120	-	2-acetolactate	at pH 8.0, temperature not specified in the publication	Oryza sativa

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Release 2023.1 (January 2023)