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Literature summary for 1.1.1.86 extracted from

  • Arfin, S.M.; Umbarger, H.E.
    Purification and properties of the acetohydroxy acid isomeroreductase of Salmonella typhimurium (1969), J. Biol. Chem., 244, 1118-1127.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
ammonium sulfate precipitation Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
2,3-dihydroxy-3-methylbutanoic acid linear noncompetitive inhibitor of both 2-acetolactate and NADPH Salmonella enterica subsp. enterica serovar Typhimurium
2-Hydroxy-2-methylbutyrate 0.01 M 45% inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2-Hydroxybutyrate 0.01 M, 48% inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2-Hydroxyisovalerate 0.01 M, 38% inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2-Methyllactate 0.001 M, 61% inhibition; competitive Salmonella enterica subsp. enterica serovar Typhimurium
2-Oxo-3-hydroxyisovalerate 0.001 mM, 58% inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2-oxoisovalerate 0.01 M, 21% inhibition Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
sedimentation equilibrium centrifugation Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-acetolactate + NADPH Salmonella enterica subsp. enterica serovar Typhimurium not reversible at pH 7.5, reversible at pH 9-10 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
2-acetolactate + NADPH Salmonella enterica subsp. enterica serovar Typhimurium enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.91
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetolactate + NADPH not reversible at pH 7.5, reversible at pH 9-10 Salmonella enterica subsp. enterica serovar Typhimurium 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Salmonella enterica subsp. enterica serovar Typhimurium 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
additional information the enzyme catalyzes the isomerization of 2-oxo-3-hydroxyisovalerate to 2-acetolactate Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?
NADPH + 2-aceto-2-hydroxybutyrate
-
Salmonella enterica subsp. enterica serovar Typhimurium NADP+ + ?
-
?
NADPH + 2-acetolactate not reversible at pH 7.5, reversible at pH 9-10 Salmonella enterica subsp. enterica serovar Typhimurium NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
?
NADPH + 2-acetolactate not reversible at pH 7.5, reversible at pH 9-10 Salmonella enterica subsp. enterica serovar Typhimurium NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
r
NADPH + 2-acetolactate alpha-keto-beta-hydroxyisovalerate is not a free intermediate Salmonella enterica subsp. enterica serovar Typhimurium NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
?
NADPH + 2-acetolactate alpha-keto-beta-hydroxyisovalerate is not a free intermediate Salmonella enterica subsp. enterica serovar Typhimurium NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
r
NADPH + 2-oxo-3-hydroxyisovalerate
-
Salmonella enterica subsp. enterica serovar Typhimurium NADP+ + alpha,beta-dihydroxyisovalerate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
2-acetolactate as substrate Salmonella enterica subsp. enterica serovar Typhimurium
9.4
-
reaction with 2,3-dihydroxyisovalerate Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8 pH 6.8: about 40% of maximal activity Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
NADPH specific for B-side of NADPH Salmonella enterica subsp. enterica serovar Typhimurium