Crystallization (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-dihydroxy-3-methylbutanoic acid | linear noncompetitive inhibitor of both 2-acetolactate and NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | |
2-Hydroxy-2-methylbutyrate | 0.01 M 45% inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
2-Hydroxybutyrate | 0.01 M, 48% inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
2-Hydroxyisovalerate | 0.01 M, 38% inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
2-Methyllactate | 0.001 M, 61% inhibition; competitive | Salmonella enterica subsp. enterica serovar Typhimurium | |
2-Oxo-3-hydroxyisovalerate | 0.001 mM, 58% inhibition | Salmonella enterica subsp. enterica serovar Typhimurium | |
2-oxoisovalerate | 0.01 M, 21% inhibition | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
220000 | - |
sedimentation equilibrium centrifugation | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | not reversible at pH 7.5, reversible at pH 9-10 | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
2-acetolactate + NADPH | Salmonella enterica subsp. enterica serovar Typhimurium | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.91 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetolactate + NADPH | not reversible at pH 7.5, reversible at pH 9-10 | Salmonella enterica subsp. enterica serovar Typhimurium | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Salmonella enterica subsp. enterica serovar Typhimurium | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
additional information | the enzyme catalyzes the isomerization of 2-oxo-3-hydroxyisovalerate to 2-acetolactate | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
NADPH + 2-aceto-2-hydroxybutyrate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | NADP+ + ? | - |
? | |
NADPH + 2-acetolactate | not reversible at pH 7.5, reversible at pH 9-10 | Salmonella enterica subsp. enterica serovar Typhimurium | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
NADPH + 2-acetolactate | not reversible at pH 7.5, reversible at pH 9-10 | Salmonella enterica subsp. enterica serovar Typhimurium | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
r | |
NADPH + 2-acetolactate | alpha-keto-beta-hydroxyisovalerate is not a free intermediate | Salmonella enterica subsp. enterica serovar Typhimurium | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
NADPH + 2-acetolactate | alpha-keto-beta-hydroxyisovalerate is not a free intermediate | Salmonella enterica subsp. enterica serovar Typhimurium | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
r | |
NADPH + 2-oxo-3-hydroxyisovalerate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | NADP+ + alpha,beta-dihydroxyisovalerate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
2-acetolactate as substrate | Salmonella enterica subsp. enterica serovar Typhimurium |
9.4 | - |
reaction with 2,3-dihydroxyisovalerate | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | pH 6.8: about 40% of maximal activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for B-side of NADPH | Salmonella enterica subsp. enterica serovar Typhimurium |