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Literature summary for 1.1.1.85 extracted from

  • Khan, M.S.; Gargiulo, S.; Soumillion, P.
    Promiscuous activity of 3-isopropylmalate dehydrogenase produced at physiological level affords Escherichia coli growth on D-malate (2020), FEBS Lett., 594, 2421-2430 .
    View publication on PubMed
Search for more literature for 1.1.1.85

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 (2R,3S)-3-isopropylmalate pH 7.5, 37°C Escherichia coli
0.301
-
 D-malate pH 7.5, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+ Escherichia coli
-
 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
(2R,3S)-3-isopropylmalate + NAD+ Escherichia coli BW25113
-
 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli BW25113
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
 Escherichia coli 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
(2R,3S)-3-isopropylmalate + NAD+
-
 Escherichia coli BW25113 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
D-malate + NAD+
-
 Escherichia coli pyruvate + NADH + CO2
-
 ?
D-malate + NAD+
-
 Escherichia coli BW25113 pyruvate + NADH + CO2
-
 ?

Synonyms

Synonyms Comment Organism
IPMDH
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
 D-malate pH 7.5, 37°C Escherichia coli
12
-
 (2R,3S)-3-isopropylmalate pH 7.5, 37°C Escherichia coli

Expression

Organism Comment Expression
Escherichia coli induced upon leucine starvation up

General Information

General Information Comment Organism
evolution for a new phenotypic trait to appear, a genetic mutation is not strictly needed, whereas environmental conditions may play an arbitrary role in enabling or not promiscuous phenotypes. D-malate metabolism is made possible by leucine deprivation, which is a priori unrelated Escherichia coli
metabolism upon leucine starvation, the physiological concentration of the enzyme raises to a level that is sufficient for metabolizing D-malate thanks to substrate promiscuity. Under these conditions, the enzyme must also play its native role in leucine biosynthesis so the enzyme is phenotypically active in two core metabolic pathways at the same time Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.36
-
 D-malate pH 7.5, 37°C Escherichia coli
1150
-
 (2R,3S)-3-isopropylmalate pH 7.5, 37°C Escherichia coli