Crystallization (Comment) | Organism |
---|---|
mutant enzyme E270A, hanging drop vapor diffusion method, using 20% (w/v) PEG 6000, 0.1 M MOPS/KOH pH 7.6, and 10% (v/v) ethanol | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E270A | the mutant exhibits largely reduced (about 1%) catalytic activity and negligible activation by K+ compared to the wild type enzyme | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, in the presence of 25 mM K+, at pH 7.6 and 20°C | Thermus thermophilus | |
0.032 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme E270A, in the presence of 25 mM K+, at pH 7.6 and 20°C | Thermus thermophilus | |
0.29 | - |
NAD+ | wild type enzyme, in the presence of 25 mM K+, at pH 7.6 and 20°C | Thermus thermophilus | |
0.66 | - |
NAD+ | mutant enzyme E270A, in the presence of 25 mM K+, at pH 7.6 and 20°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Thermus thermophilus | |
Mn2+ | activates | Thermus thermophilus | |
NH4+ | activates | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | Thermus thermophilus | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SIY4 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SIY4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPMDH | - |
Thermus thermophilus |
isopropylmalate dehydrogenase | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
(2R,3S)-3-isopropylmalate | mutant enzyme E270A, in the presence of K+, at pH 7.6 and 20°C | Thermus thermophilus | |
3.97 | - |
(2R,3S)-3-isopropylmalate | wild type enzyme, in the presence of K+, at pH 7.6 and 20°C | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus |