Protein Variants | Comment | Organism |
---|---|---|
E253A | specific activity: 4.7 unit/mg, Km: 0.016 mM (3-isopropylmalate), 0.246 mM (NAD), kcat: 14.9/sec | Bacillus subtilis |
E253F | specific activity: 7.6 unit/mg | Bacillus subtilis |
E253G | specific activity: 5.9 unit/mg | Bacillus subtilis |
E253I | specific activity: 7.4 unit/mg | Bacillus subtilis |
E253K | when a lysine is the position 253 the protein dissociates | Bacillus subtilis |
E253L | specific activity: 7.6 unit/mg, Km: 0.0122 mM (3-isopropylmalate), 0.474 mM (NAD), kcat: 13.7/sec | Bacillus subtilis |
E253Q | specific activity: 7 unit/mg, Km: 0.0189 mM (3-isopropylmalate), 0.273 mM (NAD), kcat: 9.9/sec. this mutant is more thermostable than the other mutants | Bacillus subtilis |
E253V | specific activity: 9 unit/mg, Km: 0.0244 mM (3-isopropylmalate), 0.171 mM (NAD), kcat: 12.4/sec | Bacillus subtilis |
E253W | specific activity: 5.2 unit/mg, Km: 0.0254 mM (3-isopropylmalate), 0.244 mM (NAD), kcat: 8.3/sec | Bacillus subtilis |
additional information | results show that, for residues with non-polar side chains, when positioned at residue 253, the thermostabilities of their corresponding mutants correlate positively with the relative hydrophobicities of the side chains | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
3-isopropylmalate | wild-type | Bacillus subtilis | |
0.0122 | - |
3-isopropylmalate | mutant Glu253Leu | Bacillus subtilis | |
0.016 | - |
3-isopropylmalate | mutant Glu253Ala | Bacillus subtilis | |
0.0189 | - |
3-isopropylmalate | mutant Glu253Gln | Bacillus subtilis | |
0.0244 | - |
3-isopropylmalate | mutant Glu253Val | Bacillus subtilis | |
0.0254 | - |
3-isopropylmalate | mutant Glu253Trp | Bacillus subtilis | |
0.171 | - |
NAD+ | mutant Glu253Val | Bacillus subtilis | |
0.244 | - |
NAD+ | mutant Glu253Trp | Bacillus subtilis | |
0.246 | - |
NAD+ | wild-type | Bacillus subtilis | |
0.246 | - |
NAD+ | mutant Glu253Ala | Bacillus subtilis | |
0.273 | - |
NAD+ | mutant Glu253Gln | Bacillus subtilis | |
0.474 | - |
NAD+ | mutant Glu253Leu | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Bacillus subtilis | |
Mn2+ | - |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.7 | - |
mutant E253A | Bacillus subtilis |
5.2 | - |
mutant E253W | Bacillus subtilis |
5.9 | - |
mutant E253G | Bacillus subtilis |
7 | - |
mutant E253Q | Bacillus subtilis |
7.4 | - |
mutant E253I | Bacillus subtilis |
7.6 | - |
mutant E253F | Bacillus subtilis |
7.6 | - |
mutant E253L | Bacillus subtilis |
9 | - |
mutant E253V | Bacillus subtilis |
13.9 | - |
wild-type | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-isopropylmalate + NAD+ | - |
Bacillus subtilis | 2-ketoisocaproate + NADH + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
3-isopropylmalate dehydrogenase | - |
Bacillus subtilis |
3-isopropylmalateDH | - |
Bacillus subtilis |
Bs3-isopropylmalateDH | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
all mutant Bs3-isopropylmalateDHs and the wild-type enzyme are irreversibly inactivated by heat treatments. A linear correlation between thermal stability and hydrophobicity exists for those 3-isopropylmalateDHs containing position 253 residues with hydrophobic side chains | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
3-isopropylmalate | mutant Glu253Trp | Bacillus subtilis | |
9.5 | - |
3-isopropylmalate | mutant Glu253Ala | Bacillus subtilis | |
9.9 | - |
3-isopropylmalate | mutant Glu253Gln | Bacillus subtilis | |
12.4 | - |
3-isopropylmalate | mutant Glu253Val | Bacillus subtilis | |
13.7 | - |
3-isopropylmalate | mutant Glu253Leu | Bacillus subtilis | |
14.9 | - |
3-isopropylmalate | wild-type | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bacillus subtilis |