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Literature summary for 1.1.1.85 extracted from
- Cloning of beta-isopropylmalate dehydrogenase gene from Bacillus coagulans in Escherichia coli and purification and properties of the enzyme (1984), Biochim. Biophys. Acta, 788, 267-273.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli C6000 using pBR322 as a vector plasmid | Weizmannia coagulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.5 mM, 32% inhibition | Weizmannia coagulans |  |
| Cu2+ | 0.5 mM, 77% inhibition | Weizmannia coagulans | |
| Fe2+ | 0.5 mM, 41% inhibition | Weizmannia coagulans | |
| K+ | 250 mM KCl, 23% inhibition | Weizmannia coagulans | |
| NaCl | 250 mM, 48% inhibition | Weizmannia coagulans | |
| PCMB | 0.2 mM, 90% inhibition | Weizmannia coagulans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 0.5 mM, slight activation | Weizmannia coagulans | |
| Co2+ | 0.5 mM, activates | Weizmannia coagulans | |
| Mg2+ | 0.5 mM, activates | Weizmannia coagulans | |
| Mn2+ | 0.5 mM, activates | Weizmannia coagulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
2 * 44000, monomer-dimer equilibrium in aqueous solution, SDS-PAGE | Weizmannia coagulans |
| 68000 | - |
monomer-dimer equilibrium in aqueous solution, gel filtration | Weizmannia coagulans |
| 90000 | - |
gel electrophoresis | Weizmannia coagulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Weizmannia coagulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme expressed in Escherichia coli | Weizmannia coagulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Weizmannia coagulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3S)-3-isopropylmalate + NAD+ | - |
Weizmannia coagulans | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 44000, monomer-dimer equilibrium in aqueous solution, SDS-PAGE | Weizmannia coagulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermostabilization by LiCl, NaCl, KCl and to a lesser degree with RbCl, NH4Cl and CsCl | Weizmannia coagulans |
| 60 | - |
5 min, 92% loss of activity in absence of NaCl, 6% loss of activity in presence of 1.5 M NaCl | Weizmannia coagulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Weizmannia coagulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Weizmannia coagulans | |
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