Application | Comment | Organism |
---|---|---|
analysis | the cloned 3-isopropylmalate dehydrogenase gene can be used as a genetic marker in constructing vectors in Citrobacter freundii | Citrobacter freundii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Bacillus subtilis using pLS353 as a vector and PstI as an enzyme, enzyme activities in both Escherichia coli and Bacillus subtilis transformants are 4-8fold higher than those in the present strains | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
TIT 01010 | - |
Citrobacter freundii TIT 01010 | - |
TIT 01010 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Citrobacter freundii | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Citrobacter freundii TIT 01010 | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Citrobacter freundii |