Cloned (Comment) | Organism |
---|---|
construction of a chimeric gene by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli | Bacillus subtilis |
construction of a chimeric gene by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, chimeric enzyme constructed by fusing the gene of Bacillus subtilis and Thermus thermophilus coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli | Bacillus subtilis |
hanging drop vapor diffusion method, chimeric enzyme constructed by fusing the gene of Bacillus subtilis and Thermus thermophilus coding for 3-isopropylmalate dehydrogenase, expression in Escherichia coli | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Bacillus subtilis | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Thermus thermophilus |