KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | 0.2 | 3,3-dimethyl-D-malate | - |
Pseudomonas sp. | |
0.27 | - |
NAD+ | - |
Pseudomonas sp. | |
0.33 | - |
3-(n-Propyl)malate | - |
Pseudomonas sp. | |
8.9 | 11 | D-malate | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Mn2+ or Co2+ required | Pseudomonas sp. | |
K+ | K+ or NH4+ required | Pseudomonas sp. | |
Mn2+ | Mn2+ or Co2+ required | Pseudomonas sp. | |
NH4+ | K+ or NH4+ required | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3,3-dimethylmalate + NAD+ | Pseudomonas sp. | - |
3-methyl-2-oxobutanoate + CO2 + NADH | - |
r | |
(R)-3,3-dimethylmalate + NAD+ | Pseudomonas sp. P-2 | - |
3-methyl-2-oxobutanoate + CO2 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
P-2 | - |
Pseudomonas sp. P-2 | - |
P-2 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.1 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
50 days, 0°C | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3,3-dimethylmalate + NAD+ | - |
Pseudomonas sp. | 3-methyl-2-oxobutanoate + CO2 + NADH | - |
r | |
(R)-3,3-dimethylmalate + NAD+ | - |
Pseudomonas sp. P-2 | 3-methyl-2-oxobutanoate + CO2 + NADH | - |
r | |
3-(n-propyl)malate + NAD+ | - |
Pseudomonas sp. | 2-oxopentanoate + CO2 + NADH | - |
? | |
3-(n-propyl)malate + NAD+ | - |
Pseudomonas sp. P-2 | 2-oxopentanoate + CO2 + NADH | - |
? | |
D-malate + NAD+ | - |
Pseudomonas sp. | pyruvate + CO2 + NADH | - |
? | |
D-malate + NAD+ | - |
Pseudomonas sp. P-2 | pyruvate + CO2 + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, 40% loss of activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | substrates 3,3-dimethylmalate + NAD+ | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |