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Literature summary for 1.1.1.82 extracted from

  • Jacquot, J.P.; Keryer, E.; Issakidis, E.; Decottignies, P.; Miginiac-Maslow, M.; Schmitter, J.M.; Cretin, C.
    Properties of recombinant NADP-malate dehydrogenases from Sorghum vulgare leaves expressed in Escherichia coli cells (1991), Eur. J. Biochem., 199, 47-51.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, expression vectors pJ2PCC1 and pJ2PCC2 Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 oxaloacetate recombinant enzyme cloned from expression vector pJ2PCC2 Sorghum bicolor
0.021
-
 oxaloacetate recombinant enzyme cloned from expression vector pJ2PCC1 Sorghum bicolor
0.03
-
 NADPH recombinant enzyme cloned from expression vector pJ2PCC1 Sorghum bicolor
0.043
-
 NADPH recombinant enzyme cloned from expression vector pJCPCC2 Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Sorghum bicolor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADPH
-
 Sorghum bicolor (S)-malate + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000-41000, recombinant enzyme, SDS-PAGE Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8 recombinant enzyme Sorghum bicolor