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Literature summary for 1.1.1.82 extracted from
- Properties of NADP+-malate dehydrogenase from immature pod wall of Cicer arietinum (1990), Plant Physiol. Biochem., 28, 671-678.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | 5 mM | Cicer arietinum |  |
| ATP | 5 mM | Cicer arietinum | |
| citrate | 5 mM | Cicer arietinum | |
| isocitrate | 5 mM | Cicer arietinum | |
| malate | 5 mM | Cicer arietinum | |
| NADP+ | 5 mM | Cicer arietinum | |
| oxalate | 5 mM | Cicer arietinum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
NADPH | - |
Cicer arietinum | |
| 0.029 | - |
NADP+ | - |
Cicer arietinum | |
| 0.062 | - |
oxaloacetate | - |
Cicer arietinum | |
| 2 | - |
(S)-malate | - |
Cicer arietinum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 135000 | - |
gel filtration | Cicer arietinum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate + NADPH | Cicer arietinum | under physiological conditions the enzyme functions in the direction of malate formation. Enzyme probably functions in providing the cytoplasm with reducing equivalents in the form of malate which in addition to its various roles, can be used for generation of carbon skeletons for amino acid biosynthesis | (S)-malate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cicer arietinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cicer arietinum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pod | wall | Cicer arietinum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.35 | - |
- |
Cicer arietinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Cicer arietinum | oxaloacetate + NADPH + H+ | - |
r | |
| oxaloacetate + NADPH | - |
Cicer arietinum | (S)-malate + NADP+ | - |
r | |
| oxaloacetate + NADPH | under physiological conditions the enzyme functions in the direction of malate formation. Enzyme probably functions in providing the cytoplasm with reducing equivalents in the form of malate which in addition to its various roles, can be used for generation of carbon skeletons for amino acid biosynthesis | Cicer arietinum | (S)-malate + NADP+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Cicer arietinum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | pH 5.5: about 90% of maximal activity, pH 8.0: about 40% of maximal activity | Cicer arietinum |
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Release 2023.1 (January 2023)
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