Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.82 extracted from

  • Riessland, R.; Jaenicke, R.
    Determination of the regulatory disulfide bonds of NADP-dependent malate dehydrogenase from Pisum sativum by site-directed mutagenesis (1997), Biol. Chem., 378, 983-988.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation of the inactive oxidized enzyme Pisum sativum
additional information activation by light Pisum sativum
additional information light-mediated reversible activation Pisum sativum

Protein Variants

Protein Variants Comment Organism
C23A minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions Pisum sativum
C23A/C28A minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions Pisum sativum
C23A/C28A/C206A only marginal activity in the oxidized state, does not exhibit full activity in the crude extract Pisum sativum
C28A minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions Pisum sativum
DELTAN mutant lacking the N-terminal 45 residues Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
oxaloacetate mutant enzyme C23A/C28A/C364A/C376A, oxidized form Pisum sativum
0.02
-
oxaloacetate mutant enzyme C23A, reduced form Pisum sativum
0.021
-
NADPH mutant enzyme C23A/C28A and C23A/C28A/C376A, oxidized form Pisum sativum
0.022
-
oxaloacetate mutant enzyme C23A/C28A, reduced form Pisum sativum
0.023
-
NADPH mutant enzyme C23A/C28A/C206A/C364A, oxidized form Pisum sativum
0.024
-
NADPH mutant enzyme C23A/C28A, reduced form Pisum sativum
0.024
-
oxaloacetate mutant enzyme C23A/C28A/C206A/C376A, oxidized form Pisum sativum
0.025
-
oxaloacetate mutant enzyme C23A/C28A/C206A/C364A, oxidized form and mutant DELTAN, lacking the 45 N-terminal residues Pisum sativum
0.026
-
NADPH mutant enzyme C23A/C28A/C364A and C23A/C28A/C364A/C376A, oxidized form Pisum sativum
0.027
-
oxaloacetate wild-type enzyme, reduced form and mutant enzyme C23A/C28A/C364A, oxidized form Pisum sativum
0.028
-
NADPH mutant enzyme C23A/C28A/C206A/C376A, oxidized form Pisum sativum
0.028
-
oxaloacetate mutant enzyme C23A/C28A/C206A, reduced enzyme form and mutant enzyme C23A/C28A/C376A, oxidized form Pisum sativum
0.028
-
NADPH wild-type enzyme and mutant C28A, reduced enzyme form Pisum sativum
0.029
-
NADPH mutant enzyme lacking the N-terminal 45 residues, reduced form Pisum sativum
0.03
-
NADPH mutant enzyme C23A, reduced form Pisum sativum
0.033
-
NADPH mutant enzyme C23A/C28A/C206A, oxidized form and mutant DELTAN lacking the N-terminal 45 residues, reduced form Pisum sativum
0.036
-
NADPH mutant enzyme C23A/C28A/C206A, reduced form Pisum sativum
0.039
-
NADPH
-
Pisum sativum
0.105
-
oxaloacetate mutant enzyme C23A/C28A/C206A, oxidized form Pisum sativum
0.472
-
NADPH mutant enzyme lacking the N-terminal 45 residues, oxidized form Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, mutant DELTAN, lacking the N-terminal 45 residues, SDS-PAGE Pisum sativum
42000
-
2 * 42000, wild-type enzyme and mutant enzymes C23A, C28A, C23A/C28A, C23A/C28A/C206A, C23A/C28A/C364A, C23A/C28A/C376A, C23A/C28A/C206A/C364A, C23A/C28A/C206A/C376A and C23A/C28A/C364A/C376A, SDS-PAGE Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
wild-type and mutant enzymes: C23A, C28A, C23A/C28A, C23A/C28A/C206A, C23A/C28A/C364A, C23A/C28A/C376A, C23A/C28A/C206A/C364A, C23A/C28A/C206A/C376A, C23A/C28A/C364A/C376A and mutant DELTAN, lacking the N-terminal 45 resiues
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADPH
-
Pisum sativum (S)-malate + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42000, wild-type enzyme and mutant enzymes C23A, C28A, C23A/C28A, C23A/C28A/C206A, C23A/C28A/C364A, C23A/C28A/C376A, C23A/C28A/C206A/C364A, C23A/C28A/C206A/C376A and C23A/C28A/C364A/C376A, SDS-PAGE Pisum sativum
dimer 2 * 36000, mutant DELTAN, lacking the N-terminal 45 residues, SDS-PAGE Pisum sativum