Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activation of the inactive oxidized enzyme | Pisum sativum | |
additional information | activation by light | Pisum sativum | |
additional information | light-mediated reversible activation | Pisum sativum |
Protein Variants | Comment | Organism |
---|---|---|
C23A | minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions | Pisum sativum |
C23A/C28A | minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions | Pisum sativum |
C23A/C28A/C206A | only marginal activity in the oxidized state, does not exhibit full activity in the crude extract | Pisum sativum |
C28A | minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions | Pisum sativum |
DELTAN | mutant lacking the N-terminal 45 residues | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
oxaloacetate | mutant enzyme C23A/C28A/C364A/C376A, oxidized form | Pisum sativum | |
0.02 | - |
oxaloacetate | mutant enzyme C23A, reduced form | Pisum sativum | |
0.021 | - |
NADPH | mutant enzyme C23A/C28A and C23A/C28A/C376A, oxidized form | Pisum sativum | |
0.022 | - |
oxaloacetate | mutant enzyme C23A/C28A, reduced form | Pisum sativum | |
0.023 | - |
NADPH | mutant enzyme C23A/C28A/C206A/C364A, oxidized form | Pisum sativum | |
0.024 | - |
NADPH | mutant enzyme C23A/C28A, reduced form | Pisum sativum | |
0.024 | - |
oxaloacetate | mutant enzyme C23A/C28A/C206A/C376A, oxidized form | Pisum sativum | |
0.025 | - |
oxaloacetate | mutant enzyme C23A/C28A/C206A/C364A, oxidized form and mutant DELTAN, lacking the 45 N-terminal residues | Pisum sativum | |
0.026 | - |
NADPH | mutant enzyme C23A/C28A/C364A and C23A/C28A/C364A/C376A, oxidized form | Pisum sativum | |
0.027 | - |
oxaloacetate | wild-type enzyme, reduced form and mutant enzyme C23A/C28A/C364A, oxidized form | Pisum sativum | |
0.028 | - |
NADPH | mutant enzyme C23A/C28A/C206A/C376A, oxidized form | Pisum sativum | |
0.028 | - |
oxaloacetate | mutant enzyme C23A/C28A/C206A, reduced enzyme form and mutant enzyme C23A/C28A/C376A, oxidized form | Pisum sativum | |
0.028 | - |
NADPH | wild-type enzyme and mutant C28A, reduced enzyme form | Pisum sativum | |
0.029 | - |
NADPH | mutant enzyme lacking the N-terminal 45 residues, reduced form | Pisum sativum | |
0.03 | - |
NADPH | mutant enzyme C23A, reduced form | Pisum sativum | |
0.033 | - |
NADPH | mutant enzyme C23A/C28A/C206A, oxidized form and mutant DELTAN lacking the N-terminal 45 residues, reduced form | Pisum sativum | |
0.036 | - |
NADPH | mutant enzyme C23A/C28A/C206A, reduced form | Pisum sativum | |
0.039 | - |
NADPH | - |
Pisum sativum | |
0.105 | - |
oxaloacetate | mutant enzyme C23A/C28A/C206A, oxidized form | Pisum sativum | |
0.472 | - |
NADPH | mutant enzyme lacking the N-terminal 45 residues, oxidized form | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, mutant DELTAN, lacking the N-terminal 45 residues, SDS-PAGE | Pisum sativum |
42000 | - |
2 * 42000, wild-type enzyme and mutant enzymes C23A, C28A, C23A/C28A, C23A/C28A/C206A, C23A/C28A/C364A, C23A/C28A/C376A, C23A/C28A/C206A/C364A, C23A/C28A/C206A/C376A and C23A/C28A/C364A/C376A, SDS-PAGE | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
wild-type and mutant enzymes: C23A, C28A, C23A/C28A, C23A/C28A/C206A, C23A/C28A/C364A, C23A/C28A/C376A, C23A/C28A/C206A/C364A, C23A/C28A/C206A/C376A, C23A/C28A/C364A/C376A and mutant DELTAN, lacking the N-terminal 45 resiues | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADPH | - |
Pisum sativum | (S)-malate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, wild-type enzyme and mutant enzymes C23A, C28A, C23A/C28A, C23A/C28A/C206A, C23A/C28A/C364A, C23A/C28A/C376A, C23A/C28A/C206A/C364A, C23A/C28A/C206A/C376A and C23A/C28A/C364A/C376A, SDS-PAGE | Pisum sativum |
dimer | 2 * 36000, mutant DELTAN, lacking the N-terminal 45 residues, SDS-PAGE | Pisum sativum |