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Literature summary for 1.1.1.8 extracted from

  • Fink, S.C.; Brosemer, R.W.
    Glycerol-3-phosphate dehydrogenase from the honey bee (1975), Methods Enzymol., 41B, 240-245.
    View publication on PubMed
Search for more literature for 1.1.1.8

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Apis mellifera

General Stability

General Stability Organism
dithiothreitol stabilizes Apis mellifera
EDTA stabilizes Apis mellifera

Inhibitors

Inhibitors Comment Organism Structure
dihydroxyacetone phosphate high levels of substrate, i.e. dihydroxyacetone phosphate result in inhibition Apis mellifera
L-glycerol 3-phosphate
-
 Apis mellifera
L-glycerol 3-phosphate inhibitor of bumble bee enzyme, non-competitive Bombus sp.
N-ethylmaleimide 0.1 mM inhibits enzyme over 60% Apis mellifera
p-chloromercuribenzoate 0.0001 mM inhibits enzyme over 60% Apis mellifera
phosphate competitive inhibition Bombus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADH less than 0.01 mM Apis mellifera
0.23
-
 dihydroxyacetone phosphate in MOPS buffer, 0.33 mM in Tris-histidine Apis mellifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 2 * 39000, gel filtration in guanidinium chloride Apis mellifera
73000
-
 calculated by amino acid analysis Apis mellifera
76000
-
 gel filtration Apis mellifera
76500
-
 74000-79000 Da, SDS-PAGE under non-denaturing conditions Apis mellifera
79500
-
 meniscus depletion method Apis mellifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Apis mellifera
-
 dihydroxyacetone phosphate + NADH + H+
-
 r
sn-glycerol 3-phosphate + NAD+ Bombus sp.
-
 dihydroxyacetone phosphate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Apis mellifera
-
 honey bee
-
Bombus sp.
-
 bumblebee species
-
Vespula germanica
-
 yellow jacket
-

Purification (Commentary)

Purification (Comment) Organism
-
 Apis mellifera
6 to 8 isozymes of bumblebee flight muscle enzyme Bombus sp.

Source Tissue

Source Tissue Comment Organism Textmining
muscle thoracic flight muscle Apis mellifera
-
muscle thoracic flight muscle Bombus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
340
-
-
 Apis mellifera

Storage Stability

Storage Stability Organism
2°C, crystals stable at least a month in 0.1 M Tris buffer, pH 7.6 with added EDTA, dithiothreitol and ammonium sulfate Apis mellifera
crystals, 2°C in 0.1M Tris, 10 mM EDTA, 2 mM dithiothreitol, pH 7.6, (NH4)2SO4 in final concentration of 1.7 M Apis mellifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
 Apis mellifera dihydroxyacetone phosphate + NADH + H+
-
 r
sn-glycerol 3-phosphate + NAD+
-
 Bombus sp. dihydroxyacetone phosphate + NADH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 39000, gel filtration in guanidinium chloride Apis mellifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
21
-
 15 min stable Apis mellifera
21
-
 stable at pH 4.8-9.9 for 15 min Apis mellifera
55
-
 5 min stable in Tris-buffer plus EDTA and bovine serum albumin Apis mellifera
61
-
 complete inactivation after 5 min Apis mellifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
 broad pH optimum around pH 6.6 Apis mellifera

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.8 9.9 stable for 15 min at 21°C Apis mellifera
4.8 9.9 stable at 21°C for 15 min Apis mellifera

Cofactor

Cofactor Comment Organism Structure
additional information deamino-NAD+ 68% as effective as NAD+ Apis mellifera
additional information 3-acetylpyridine-NAD+ 1.5% as effective as NAD+ Apis mellifera
NAD+
-
 Apis mellifera
NAD+
-
 Bombus sp.
NAD+
-
 Vespula germanica
NADH
-
 Vespula germanica
NADH 2 mol per mol enzyme Apis mellifera