Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.79 extracted from

  • Fukuda, H.; Moriguchi, M.; Kimura, A.; Tochikura, T.
    Purification and properties of glyoxylate reductase from Neurospora crassa (1981), Agric. Biol. Chem., 45, 1153-1158.
No PubMed abstract available
Search for more literature for 1.1.1.79

Inhibitors

Inhibitors Comment Organism Structure
nitrate 25 mM, competitive vs. glyoxylate Neurospora crassa
p-chloromercuribenzoate 1 mM, 70% inhibition Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 NADPH
-
 Neurospora crassa
0.085
-
 Hydroxypyruvate
-
 Neurospora crassa
0.23
-
 NADH
-
 Neurospora crassa
5.7
-
 glyoxylate
-
 Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glyoxylate + NAD(P)H Neurospora crassa
-
 glycolate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme 2, protamine sulfate, DEAE-cellulose, hydroxylapatite, Sephadex G-150, DEAE-cellulose, phospho-cellulose Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
108.1
-
 isoenzyme 2 Neurospora crassa

Storage Stability

Storage Stability Organism
4°C, alkaline pH, no loss of activity after 1 week, at pH 7-11, 20% loss of activity Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + NAD(P)H
-
 Neurospora crassa glycolate + NAD(P)+
-
 ?
glyoxylate + NAD(P)H isoenzyme 2, 16% activity with NADH Neurospora crassa glycolate + NAD(P)+
-
 ?
glyoxylate + NADH + H+
-
 Neurospora crassa glycolate + NAD+
-
 r
hydroxypyruvate + NAD(P)H + H+ 25 mM hydroxypyruvate, 68% of the activity with glyoxylate, 2.5 mM hydroxypyruvate, isoenzyme 2, 332% of the activity of glyoxylate Neurospora crassa glycerate + NAD(P)+
-
 ?
phenylpyruvate + NAD(P)H isoenzyme 2, 6% activity of glyoxylate Neurospora crassa phenyllactate + NAD(P)+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 60% loss of activity after 10 min, activity is completely lost above 60°C Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 reduction of hydroxypyruvate Neurospora crassa
6.7
-
 reduction of glyoxylate Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
NAD(P)H NADPH is preferred Neurospora crassa
NADPH NADPH is preferred Neurospora crassa