BRENDA - Enzyme Database
show all sequences of 1.1.1.71

Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols

Wu, X.; Zhang, C.; Orita, I.; Imanaka, T.; Fukui, T.; Xing, X.H.; Appl. Environ. Microbiol. 79, 2209-2217 (2013)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli Rosetta(DE3) cells
Thermococcus kodakarensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
-
Thermococcus kodakarensis
Ca2+
-
Thermococcus kodakarensis
Co2+
-
Thermococcus kodakarensis
Cu2+
-
Thermococcus kodakarensis
Mg2+
slight inhibition
Thermococcus kodakarensis
additional information
dithiothreitol and EDTA have no effect on either oxidative or reductive activity
Thermococcus kodakarensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.066
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.089
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.105
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.113
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.113
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.127
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
30.2
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
61.3
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
244
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
gel filtration
Thermococcus kodakarensis
31000
-
1 * 31000, SDS-PAGE
Thermococcus kodakarensis
31464
-
-
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(RS)-1-phenylethanol + NAD+
Thermococcus kodakarensis
100% activity
1-phenylethanone + NADH + H+
-
-
?
(RS)-1-phenylethanol + NADP+
Thermococcus kodakarensis
100% activity
1-phenylethanone + NADPH + H+
-
-
?
1-(2-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
11.3% activity compared to (RS)-1-phenylethanol
1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(2-chlorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
16.4% activity compared to (RS)-1-phenylethanol
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(3-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
315% activity compared to (RS)-1-phenylethanol
1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(3-chlorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
205% activity compared to (RS)-1-phenylethanol
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(4-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
167% activity compared to (RS)-1-phenylethanol
1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(4-chlorophenyl)ethanone + NAD(P)+
Thermococcus kodakarensis
151% activity compared to (RS)-1-phenylethanol
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(4-fluorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
119% activity compared to (RS)-1-phenylethanol
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
-
?
1-(4-methylphenyl)ethanone + NAD(P)+
Thermococcus kodakarensis
189% activity compared to (RS)-1-phenylethanol
1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
-
?
1-butanol + NAD(P)+
Thermococcus kodakarensis
2.3% activity compared to (RS)-1-phenylethanol
butanal + NAD(P)H + H+
-
-
?
1-heptanol + NAD(P)+
Thermococcus kodakarensis
3.3% activity compared to (RS)-1-phenylethanol
heptanal + NAD(P)H + H+
-
-
?
1-hexanol + NAD(P)+
Thermococcus kodakarensis
2.8% activity compared to (RS)-1-phenylethanol
hexanal + NAD(P)H + H+
-
-
?
1-octanol + NAD(P)+
Thermococcus kodakarensis
2.3% activity compared to (RS)-1-phenylethanol
octanal + NAD(P)H + H+
-
-
?
1-pentanol + NAD(P)+
Thermococcus kodakarensis
2.8% activity compared to (RS)-1-phenylethanol
pentanal + NAD(P)H + H+
-
-
?
1-phenyl-1,2-ethanediol + NAD(P)+
Thermococcus kodakarensis
3.4% activity compared to (RS)-1-phenylethanol
?
-
-
?
1-phenyl-1-propanol + NAD(P)+
Thermococcus kodakarensis
13.1% activity compared to (RS)-1-phenylethanol
1-phenylpropionaldehyde + NAD(P)H + H+
-
-
?
1-phenyl-2-propanol + NAD(P)+
Thermococcus kodakarensis
39.1% activity compared to (RS)-1-phenylethanol
1-phenylpropan-2-one + NAD(P)H + H+
-
-
?
1-propanol + NAD(P)+
Thermococcus kodakarensis
1.2% activity compared to (RS)-1-phenylethanol
propionaldehyde + NAD(P)H + H+
-
-
?
2,2,2-trifluoroacetophenone + NAD(P)H + H+
Thermococcus kodakarensis
180% activity compared to acetoin
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
-
?
2-bromoacetophenone + NAD(P)H + H+
Thermococcus kodakarensis
8.7% activity compared to acetoin
1-(2-bromophenyl)ethanone + NAD(P)+
-
-
?
2-butanol + NAD(P)+
Thermococcus kodakarensis
22.3% activity compared to (RS)-1-phenylethanol
butan-2-one + NAD(P)H + H+
-
-
?
2-chloro-1-phenylethanol + NAD(P)+
Thermococcus kodakarensis
0.3% activity compared to (RS)-1-phenylethanol
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
-
?
2-heptanol + NAD(P)+
Thermococcus kodakarensis
61.6% activity compared to (RS)-1-phenylethanol
heptan-2-one + NAD(P)H + H+
-
-
?
2-hexanol + NAD(P)+
Thermococcus kodakarensis
38.7% activity compared to (RS)-1-phenylethanol
hexan-2-one + NAD(P)H + H+
-
-
?
2-hexanone + NAD(P)H + H+
Thermococcus kodakarensis
3.6% activity compared to acetoin
hexan-2-ol + NAD(P)+
-
-
?
2-octanol + NAD(P)+
Thermococcus kodakarensis
45.8% activity compared to (RS)-1-phenylethanol
octan-2-one + NAD(P)H + H+
-
-
?
2-pentanol + NAD(P)+
Thermococcus kodakarensis
30.2% activity compared to (RS)-1-phenylethanol
pentan-2-one + NAD(P)H + H+
-
-
?
2-phenylethanol + NAD(P)+
Thermococcus kodakarensis
0.3% activity compared to (RS)-1-phenylethanol
phenylacetaldehyde + NAD(P)H + H+
-
-
?
2-propanol + NAD(P)+
Thermococcus kodakarensis
5.3% activity compared to (RS)-1-phenylethanol
propan-2-one + NAD(P)H + H+
-
-
?
acetoin + NAD(P)+
Thermococcus kodakarensis
4.5% activity compared to (RS)-1-phenylethanol
2,3-butanediol + NAD(P)H + H+
-
-
?
acetoin + NAD(P)H + H+
Thermococcus kodakarensis
100% activity
butan-2,3-dione + NAD(P)H + H+
-
-
?
acetone + NAD(P)H + H+
Thermococcus kodakarensis
4.1% activity compared to acetoin
propan-2-ol + NAD(P)+
-
-
?
cyclohexanol + NAD(P)+
Thermococcus kodakarensis
52.3% activity compared to (RS)-1-phenylethanol
cyclohexanone + NAD(P)H + H+
-
-
?
cyclohexanone + NAD(P)H + H+
Thermococcus kodakarensis
19.1% activity compared to acetoin
cyclohexanol + NAD(P)+
-
-
?
D-arabinose + NAD(P)+
Thermococcus kodakarensis
21.4% activity compared to (RS)-1-phenylethanol
?
-
-
?
dihydroxyacetone + NAD(P)H + H+
Thermococcus kodakarensis
34.8% activity compared to acetoin
?
-
-
?
ethanol + NAD(P)+
Thermococcus kodakarensis
0.5% activity compared to (RS)-1-phenylethanol
acetaldehyde + NAD(P)H + H+
-
-
?
ethyl pyruvate + NAD(P)H + H+
Thermococcus kodakarensis
294% activity compared to acetoin
?
-
-
?
hexanal + NAD(P)H + H+
Thermococcus kodakarensis
12.9% activity compared to acetoin
hexan-1-ol + NAD(P)+
-
-
?
L-arabinose + NAD(P)+
Thermococcus kodakarensis
5.2% activity compared to (RS)-1-phenylethanol
?
-
-
?
meso-2,3-butanediol + NAD+
Thermococcus kodakarensis
272% activity compared to (RS)-1-phenylethanol
?
-
-
?
meso-2,3-butanediol + NADP+
Thermococcus kodakarensis
272% activity compared to (RS)-1-phenylethanol
?
-
-
?
methanol + NAD(P)+
Thermococcus kodakarensis
0.2% activity compared to (RS)-1-phenylethanol
formaldehyde + NAD(P)H + H+
-
-
?
methyl benzoylformate + NAD(P)H + H+
Thermococcus kodakarensis
67% activity compared to acetoin
?
-
-
?
additional information
Thermococcus kodakarensis
no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone
?
-
-
-
phenylacetaldehyde + NAD(P)H + H+
Thermococcus kodakarensis
18.6% activity compared to acetoin
2-phenylethan-1-ol + NAD(P)+
-
-
?
pyruvic aldehyde + NAD(P)H + H+
Thermococcus kodakarensis
328% activity compared to acetoin
?
-
-
?
Organic Solvent Stability
Organic Solvent
Commentary
Organism
2-propanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
Acetone
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
dimethylfluoride
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
DMSO
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
Ethanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
ethylacetate
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
hexane
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
Methanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
octane
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
octanol
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
Organism
Organism
UniProt
Commentary
Textmining
Thermococcus kodakarensis
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Q sepharose column chromatography
Thermococcus kodakarensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(RS)-1-phenylethanol + NAD+
100% activity
735486
Thermococcus kodakarensis
1-phenylethanone + NADH + H+
-
-
-
?
(RS)-1-phenylethanol + NADP+
100% activity
735486
Thermococcus kodakarensis
1-phenylethanone + NADPH + H+
-
-
-
?
1-(2-bromophenyl)ethanol + NAD(P)+
11.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(2-chlorophenyl)ethanol + NAD(P)+
16.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(3-bromophenyl)ethanol + NAD(P)+
315% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(3-chlorophenyl)ethanol + NAD(P)+
205% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(4-bromophenyl)ethanol + NAD(P)+
167% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(4-chlorophenyl)ethanone + NAD(P)+
151% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(4-fluorophenyl)ethanol + NAD(P)+
119% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
-
-
?
1-(4-methylphenyl)ethanone + NAD(P)+
189% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
-
-
?
1-butanol + NAD(P)+
2.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
butanal + NAD(P)H + H+
-
-
-
?
1-heptanol + NAD(P)+
3.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
heptanal + NAD(P)H + H+
-
-
-
?
1-hexanol + NAD(P)+
2.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
hexanal + NAD(P)H + H+
-
-
-
?
1-octanol + NAD(P)+
2.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
octanal + NAD(P)H + H+
-
-
-
?
1-pentanol + NAD(P)+
2.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
pentanal + NAD(P)H + H+
-
-
-
?
1-phenyl-1,2-ethanediol + NAD(P)+
3.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1-phenyl-1-propanol + NAD(P)+
13.1% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-phenylpropionaldehyde + NAD(P)H + H+
-
-
-
?
1-phenyl-2-propanol + NAD(P)+
39.1% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-phenylpropan-2-one + NAD(P)H + H+
-
-
-
?
1-propanol + NAD(P)+
1.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
propionaldehyde + NAD(P)H + H+
-
-
-
?
2,2,2-trifluoroacetophenone + NAD(P)H + H+
180% activity compared to acetoin
735486
Thermococcus kodakarensis
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
-
-
?
2-bromoacetophenone + NAD(P)H + H+
8.7% activity compared to acetoin
735486
Thermococcus kodakarensis
1-(2-bromophenyl)ethanone + NAD(P)+
-
-
-
?
2-butanol + NAD(P)+
22.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
butan-2-one + NAD(P)H + H+
-
-
-
?
2-chloro-1-phenylethanol + NAD(P)+
0.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
-
-
?
2-heptanol + NAD(P)+
61.6% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
heptan-2-one + NAD(P)H + H+
-
-
-
?
2-hexanol + NAD(P)+
38.7% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
hexan-2-one + NAD(P)H + H+
-
-
-
?
2-hexanone + NAD(P)H + H+
3.6% activity compared to acetoin
735486
Thermococcus kodakarensis
hexan-2-ol + NAD(P)+
-
-
-
?
2-octanol + NAD(P)+
45.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
octan-2-one + NAD(P)H + H+
-
-
-
?
2-pentanol + NAD(P)+
30.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
pentan-2-one + NAD(P)H + H+
-
-
-
?
2-phenylethanol + NAD(P)+
0.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
phenylacetaldehyde + NAD(P)H + H+
-
-
-
?
2-propanol + NAD(P)+
5.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
propan-2-one + NAD(P)H + H+
-
-
-
?
acetoin + NAD(P)+
4.5% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
2,3-butanediol + NAD(P)H + H+
-
-
-
?
acetoin + NAD(P)H + H+
100% activity
735486
Thermococcus kodakarensis
butan-2,3-dione + NAD(P)H + H+
-
-
-
?
acetone + NAD(P)H + H+
4.1% activity compared to acetoin
735486
Thermococcus kodakarensis
propan-2-ol + NAD(P)+
-
-
-
?
cyclohexanol + NAD(P)+
52.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
cyclohexanone + NAD(P)H + H+
-
-
-
?
cyclohexanone + NAD(P)H + H+
19.1% activity compared to acetoin
735486
Thermococcus kodakarensis
cyclohexanol + NAD(P)+
-
-
-
?
D-arabinose + NAD(P)+
21.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
dihydroxyacetone + NAD(P)H + H+
34.8% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
ethanol + NAD(P)+
0.5% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
acetaldehyde + NAD(P)H + H+
-
-
-
?
ethyl pyruvate + NAD(P)H + H+
294% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
hexanal + NAD(P)H + H+
12.9% activity compared to acetoin
735486
Thermococcus kodakarensis
hexan-1-ol + NAD(P)+
-
-
-
?
L-arabinose + NAD(P)+
5.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
meso-2,3-butanediol + NAD+
272% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
meso-2,3-butanediol + NADP+
272% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
methanol + NAD(P)+
0.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
formaldehyde + NAD(P)H + H+
-
-
-
?
methyl benzoylformate + NAD(P)H + H+
67% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
additional information
no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone
735486
Thermococcus kodakarensis
?
-
-
-
-
phenylacetaldehyde + NAD(P)H + H+
18.6% activity compared to acetoin
735486
Thermococcus kodakarensis
2-phenylethan-1-ol + NAD(P)+
-
-
-
?
pyruvic aldehyde + NAD(P)H + H+
328% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 31000, SDS-PAGE; 1 * 31464, calculated from amino acid sequence
Thermococcus kodakarensis
Synonyms
Synonyms
Commentary
Organism
ADH
-
Thermococcus kodakarensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Thermococcus kodakarensis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
95
-
the highly thermostable enzyme has a half-life of 4.5 h at 95°C and 16.2 h at 85°C
Thermococcus kodakarensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10.4
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
10.5
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
13.7
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
15.6
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
17.3
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
30
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
30.8
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
35.7
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
43.8
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3
-
reduction reaction
Thermococcus kodakarensis
9
-
oxidation reaction
Thermococcus kodakarensis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Thermococcus kodakarensis
NADP+
-
Thermococcus kodakarensis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta(DE3) cells
Thermococcus kodakarensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Thermococcus kodakarensis
NADP+
-
Thermococcus kodakarensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
-
Thermococcus kodakarensis
Ca2+
-
Thermococcus kodakarensis
Co2+
-
Thermococcus kodakarensis
Cu2+
-
Thermococcus kodakarensis
Mg2+
slight inhibition
Thermococcus kodakarensis
additional information
dithiothreitol and EDTA have no effect on either oxidative or reductive activity
Thermococcus kodakarensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.066
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.089
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.105
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.113
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.113
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.127
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
30.2
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
61.3
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
244
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
gel filtration
Thermococcus kodakarensis
31000
-
1 * 31000, SDS-PAGE
Thermococcus kodakarensis
31464
-
-
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(RS)-1-phenylethanol + NAD+
Thermococcus kodakarensis
100% activity
1-phenylethanone + NADH + H+
-
-
?
(RS)-1-phenylethanol + NADP+
Thermococcus kodakarensis
100% activity
1-phenylethanone + NADPH + H+
-
-
?
1-(2-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
11.3% activity compared to (RS)-1-phenylethanol
1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(2-chlorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
16.4% activity compared to (RS)-1-phenylethanol
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(3-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
315% activity compared to (RS)-1-phenylethanol
1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(3-chlorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
205% activity compared to (RS)-1-phenylethanol
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(4-bromophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
167% activity compared to (RS)-1-phenylethanol
1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(4-chlorophenyl)ethanone + NAD(P)+
Thermococcus kodakarensis
151% activity compared to (RS)-1-phenylethanol
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
-
?
1-(4-fluorophenyl)ethanol + NAD(P)+
Thermococcus kodakarensis
119% activity compared to (RS)-1-phenylethanol
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
-
?
1-(4-methylphenyl)ethanone + NAD(P)+
Thermococcus kodakarensis
189% activity compared to (RS)-1-phenylethanol
1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
-
?
1-butanol + NAD(P)+
Thermococcus kodakarensis
2.3% activity compared to (RS)-1-phenylethanol
butanal + NAD(P)H + H+
-
-
?
1-heptanol + NAD(P)+
Thermococcus kodakarensis
3.3% activity compared to (RS)-1-phenylethanol
heptanal + NAD(P)H + H+
-
-
?
1-hexanol + NAD(P)+
Thermococcus kodakarensis
2.8% activity compared to (RS)-1-phenylethanol
hexanal + NAD(P)H + H+
-
-
?
1-octanol + NAD(P)+
Thermococcus kodakarensis
2.3% activity compared to (RS)-1-phenylethanol
octanal + NAD(P)H + H+
-
-
?
1-pentanol + NAD(P)+
Thermococcus kodakarensis
2.8% activity compared to (RS)-1-phenylethanol
pentanal + NAD(P)H + H+
-
-
?
1-phenyl-1,2-ethanediol + NAD(P)+
Thermococcus kodakarensis
3.4% activity compared to (RS)-1-phenylethanol
?
-
-
?
1-phenyl-1-propanol + NAD(P)+
Thermococcus kodakarensis
13.1% activity compared to (RS)-1-phenylethanol
1-phenylpropionaldehyde + NAD(P)H + H+
-
-
?
1-phenyl-2-propanol + NAD(P)+
Thermococcus kodakarensis
39.1% activity compared to (RS)-1-phenylethanol
1-phenylpropan-2-one + NAD(P)H + H+
-
-
?
1-propanol + NAD(P)+
Thermococcus kodakarensis
1.2% activity compared to (RS)-1-phenylethanol
propionaldehyde + NAD(P)H + H+
-
-
?
2,2,2-trifluoroacetophenone + NAD(P)H + H+
Thermococcus kodakarensis
180% activity compared to acetoin
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
-
?
2-bromoacetophenone + NAD(P)H + H+
Thermococcus kodakarensis
8.7% activity compared to acetoin
1-(2-bromophenyl)ethanone + NAD(P)+
-
-
?
2-butanol + NAD(P)+
Thermococcus kodakarensis
22.3% activity compared to (RS)-1-phenylethanol
butan-2-one + NAD(P)H + H+
-
-
?
2-chloro-1-phenylethanol + NAD(P)+
Thermococcus kodakarensis
0.3% activity compared to (RS)-1-phenylethanol
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
-
?
2-heptanol + NAD(P)+
Thermococcus kodakarensis
61.6% activity compared to (RS)-1-phenylethanol
heptan-2-one + NAD(P)H + H+
-
-
?
2-hexanol + NAD(P)+
Thermococcus kodakarensis
38.7% activity compared to (RS)-1-phenylethanol
hexan-2-one + NAD(P)H + H+
-
-
?
2-hexanone + NAD(P)H + H+
Thermococcus kodakarensis
3.6% activity compared to acetoin
hexan-2-ol + NAD(P)+
-
-
?
2-octanol + NAD(P)+
Thermococcus kodakarensis
45.8% activity compared to (RS)-1-phenylethanol
octan-2-one + NAD(P)H + H+
-
-
?
2-pentanol + NAD(P)+
Thermococcus kodakarensis
30.2% activity compared to (RS)-1-phenylethanol
pentan-2-one + NAD(P)H + H+
-
-
?
2-phenylethanol + NAD(P)+
Thermococcus kodakarensis
0.3% activity compared to (RS)-1-phenylethanol
phenylacetaldehyde + NAD(P)H + H+
-
-
?
2-propanol + NAD(P)+
Thermococcus kodakarensis
5.3% activity compared to (RS)-1-phenylethanol
propan-2-one + NAD(P)H + H+
-
-
?
acetoin + NAD(P)+
Thermococcus kodakarensis
4.5% activity compared to (RS)-1-phenylethanol
2,3-butanediol + NAD(P)H + H+
-
-
?
acetoin + NAD(P)H + H+
Thermococcus kodakarensis
100% activity
butan-2,3-dione + NAD(P)H + H+
-
-
?
acetone + NAD(P)H + H+
Thermococcus kodakarensis
4.1% activity compared to acetoin
propan-2-ol + NAD(P)+
-
-
?
cyclohexanol + NAD(P)+
Thermococcus kodakarensis
52.3% activity compared to (RS)-1-phenylethanol
cyclohexanone + NAD(P)H + H+
-
-
?
cyclohexanone + NAD(P)H + H+
Thermococcus kodakarensis
19.1% activity compared to acetoin
cyclohexanol + NAD(P)+
-
-
?
D-arabinose + NAD(P)+
Thermococcus kodakarensis
21.4% activity compared to (RS)-1-phenylethanol
?
-
-
?
dihydroxyacetone + NAD(P)H + H+
Thermococcus kodakarensis
34.8% activity compared to acetoin
?
-
-
?
ethanol + NAD(P)+
Thermococcus kodakarensis
0.5% activity compared to (RS)-1-phenylethanol
acetaldehyde + NAD(P)H + H+
-
-
?
ethyl pyruvate + NAD(P)H + H+
Thermococcus kodakarensis
294% activity compared to acetoin
?
-
-
?
hexanal + NAD(P)H + H+
Thermococcus kodakarensis
12.9% activity compared to acetoin
hexan-1-ol + NAD(P)+
-
-
?
L-arabinose + NAD(P)+
Thermococcus kodakarensis
5.2% activity compared to (RS)-1-phenylethanol
?
-
-
?
meso-2,3-butanediol + NAD+
Thermococcus kodakarensis
272% activity compared to (RS)-1-phenylethanol
?
-
-
?
meso-2,3-butanediol + NADP+
Thermococcus kodakarensis
272% activity compared to (RS)-1-phenylethanol
?
-
-
?
methanol + NAD(P)+
Thermococcus kodakarensis
0.2% activity compared to (RS)-1-phenylethanol
formaldehyde + NAD(P)H + H+
-
-
?
methyl benzoylformate + NAD(P)H + H+
Thermococcus kodakarensis
67% activity compared to acetoin
?
-
-
?
additional information
Thermococcus kodakarensis
no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone
?
-
-
-
phenylacetaldehyde + NAD(P)H + H+
Thermococcus kodakarensis
18.6% activity compared to acetoin
2-phenylethan-1-ol + NAD(P)+
-
-
?
pyruvic aldehyde + NAD(P)H + H+
Thermococcus kodakarensis
328% activity compared to acetoin
?
-
-
?
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
2-propanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
Acetone
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
dimethylfluoride
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
DMSO
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
Ethanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
ethylacetate
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
hexane
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
Methanol
high activity (90% of the activity without solvents) is observed in the presence of 20% (v/v)
Thermococcus kodakarensis
octane
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
octanol
high activity (90% of the activity without solvents) is observed in the presence of 50% (v/v)
Thermococcus kodakarensis
Purification (Commentary) (protein specific)
Commentary
Organism
Q sepharose column chromatography
Thermococcus kodakarensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(RS)-1-phenylethanol + NAD+
100% activity
735486
Thermococcus kodakarensis
1-phenylethanone + NADH + H+
-
-
-
?
(RS)-1-phenylethanol + NADP+
100% activity
735486
Thermococcus kodakarensis
1-phenylethanone + NADPH + H+
-
-
-
?
1-(2-bromophenyl)ethanol + NAD(P)+
11.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(2-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(2-chlorophenyl)ethanol + NAD(P)+
16.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(2-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(3-bromophenyl)ethanol + NAD(P)+
315% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(3-chlorophenyl)ethanol + NAD(P)+
205% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(3-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(4-bromophenyl)ethanol + NAD(P)+
167% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-bromophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(4-chlorophenyl)ethanone + NAD(P)+
151% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-chlorophenyl)ethanone + NAD(P)H + H+
-
-
-
?
1-(4-fluorophenyl)ethanol + NAD(P)+
119% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-fluorophenyl)ethanol + NAD(P)H + H+
-
-
-
?
1-(4-methylphenyl)ethanone + NAD(P)+
189% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-(4-methylphenyl)ethanol + NAD(P)H + H+
-
-
-
?
1-butanol + NAD(P)+
2.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
butanal + NAD(P)H + H+
-
-
-
?
1-heptanol + NAD(P)+
3.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
heptanal + NAD(P)H + H+
-
-
-
?
1-hexanol + NAD(P)+
2.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
hexanal + NAD(P)H + H+
-
-
-
?
1-octanol + NAD(P)+
2.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
octanal + NAD(P)H + H+
-
-
-
?
1-pentanol + NAD(P)+
2.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
pentanal + NAD(P)H + H+
-
-
-
?
1-phenyl-1,2-ethanediol + NAD(P)+
3.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
1-phenyl-1-propanol + NAD(P)+
13.1% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-phenylpropionaldehyde + NAD(P)H + H+
-
-
-
?
1-phenyl-2-propanol + NAD(P)+
39.1% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
1-phenylpropan-2-one + NAD(P)H + H+
-
-
-
?
1-propanol + NAD(P)+
1.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
propionaldehyde + NAD(P)H + H+
-
-
-
?
2,2,2-trifluoroacetophenone + NAD(P)H + H+
180% activity compared to acetoin
735486
Thermococcus kodakarensis
2,2,2-trifluoro-1-phenylethan-1-ol + NAD(P)+
-
-
-
?
2-bromoacetophenone + NAD(P)H + H+
8.7% activity compared to acetoin
735486
Thermococcus kodakarensis
1-(2-bromophenyl)ethanone + NAD(P)+
-
-
-
?
2-butanol + NAD(P)+
22.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
butan-2-one + NAD(P)H + H+
-
-
-
?
2-chloro-1-phenylethanol + NAD(P)+
0.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
2-chloro-1-phenylethan-1-one + NAD(P)H + H+
-
-
-
?
2-heptanol + NAD(P)+
61.6% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
heptan-2-one + NAD(P)H + H+
-
-
-
?
2-hexanol + NAD(P)+
38.7% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
hexan-2-one + NAD(P)H + H+
-
-
-
?
2-hexanone + NAD(P)H + H+
3.6% activity compared to acetoin
735486
Thermococcus kodakarensis
hexan-2-ol + NAD(P)+
-
-
-
?
2-octanol + NAD(P)+
45.8% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
octan-2-one + NAD(P)H + H+
-
-
-
?
2-pentanol + NAD(P)+
30.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
pentan-2-one + NAD(P)H + H+
-
-
-
?
2-phenylethanol + NAD(P)+
0.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
phenylacetaldehyde + NAD(P)H + H+
-
-
-
?
2-propanol + NAD(P)+
5.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
propan-2-one + NAD(P)H + H+
-
-
-
?
acetoin + NAD(P)+
4.5% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
2,3-butanediol + NAD(P)H + H+
-
-
-
?
acetoin + NAD(P)H + H+
100% activity
735486
Thermococcus kodakarensis
butan-2,3-dione + NAD(P)H + H+
-
-
-
?
acetone + NAD(P)H + H+
4.1% activity compared to acetoin
735486
Thermococcus kodakarensis
propan-2-ol + NAD(P)+
-
-
-
?
cyclohexanol + NAD(P)+
52.3% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
cyclohexanone + NAD(P)H + H+
-
-
-
?
cyclohexanone + NAD(P)H + H+
19.1% activity compared to acetoin
735486
Thermococcus kodakarensis
cyclohexanol + NAD(P)+
-
-
-
?
D-arabinose + NAD(P)+
21.4% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
dihydroxyacetone + NAD(P)H + H+
34.8% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
ethanol + NAD(P)+
0.5% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
acetaldehyde + NAD(P)H + H+
-
-
-
?
ethyl pyruvate + NAD(P)H + H+
294% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
hexanal + NAD(P)H + H+
12.9% activity compared to acetoin
735486
Thermococcus kodakarensis
hexan-1-ol + NAD(P)+
-
-
-
?
L-arabinose + NAD(P)+
5.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
meso-2,3-butanediol + NAD+
272% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
meso-2,3-butanediol + NADP+
272% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
?
-
-
-
?
methanol + NAD(P)+
0.2% activity compared to (RS)-1-phenylethanol
735486
Thermococcus kodakarensis
formaldehyde + NAD(P)H + H+
-
-
-
?
methyl benzoylformate + NAD(P)H + H+
67% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
additional information
no activity with D-arabinose, L-arabinose, acetophenone, and 4-chloroacetophenone
735486
Thermococcus kodakarensis
?
-
-
-
-
phenylacetaldehyde + NAD(P)H + H+
18.6% activity compared to acetoin
735486
Thermococcus kodakarensis
2-phenylethan-1-ol + NAD(P)+
-
-
-
?
pyruvic aldehyde + NAD(P)H + H+
328% activity compared to acetoin
735486
Thermococcus kodakarensis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 31000, SDS-PAGE; 1 * 31464, calculated from amino acid sequence
Thermococcus kodakarensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Thermococcus kodakarensis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
95
-
the highly thermostable enzyme has a half-life of 4.5 h at 95°C and 16.2 h at 85°C
Thermococcus kodakarensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10.4
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
10.5
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
13.7
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
15.6
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
17.3
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
30
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
30.8
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
35.7
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
43.8
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3
-
reduction reaction
Thermococcus kodakarensis
9
-
oxidation reaction
Thermococcus kodakarensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.15
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.45
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.71
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
117
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
149
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
153
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
159
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
243
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
265
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.15
-
(RS)-1-phenylethanol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.45
-
acetoin
with NADH as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
0.71
-
meso-2,3-butanediol
with NAD+ as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
117
-
NAD+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
149
-
NADH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
153
-
NADPH
with acetoin as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
159
-
NADP+
with (RS)-1-phenylethanol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
243
-
NAD+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
265
-
NADP+
with meso-2,3-butanediol as cosubstrate, at pH 6.0 and 70°C
Thermococcus kodakarensis
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739830
Holec
-
P450 BM3 monooxygenase as an e ...
Equus caballus, Ralstonia sp., Thermoanaerobacter brockii
Adv. Synth. Catal.
358
1810-1819
2016
-
-
-
-
-
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3
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1
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4
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3
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6
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6
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1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738540
Zheng
Cofactor specificity of the bi ...
Hungateiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
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6
-
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6
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3
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3
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-
5
-
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-
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-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
4
4
740291
Lundova
Molecular and biochemical char ...
Homo sapiens
Chem. Biol. Interact.
234
178-187
2015
-
-
1
-
-
-
-
-
2
-
1
1
-
3
-
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1
-
-
6
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8
1
4
-
-
-
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1
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-
-
-
1
1
-
-
-
-
-
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2
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1
1
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1
-
6
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741095
Brummund
-
Process Development for biocat ...
Rhodococcus ruber
Org. Process Res. Dev.
19
1590-1595
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
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1
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1
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-
-
-
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2
-
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-
2
-
-
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-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737536
Elleuche
Group III alcohol dehydrogenas ...
Pectobacterium atrosepticum, Pectobacterium atrosepticum DSM18077
Appl. Microbiol. Biotechnol.
98
4041-4051
2014
-
-
1
-
5
-
4
-
-
1
2
-
-
2
-
-
1
-
-
-
-
-
15
1
3
1
1
1
-
1
1
-
1
-
1
-
-
-
1
1
-
5
-
-
4
-
-
-
1
2
-
-
-
-
1
-
-
-
-
15
1
1
1
1
-
1
1
-
1
-
-
-
-
-
-
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
-
-
1
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
1
1
3
2
5
6
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium salinarum NRC 1, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
-
-
2
-
-
-
-
10
-
3
1
-
8
4
-
-
1
-
-
-
-
2
26
1
3
1
-
-
-
4
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
10
-
3
1
-
8
-
-
1
-
-
-
2
26
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
735486
Wu
Thermostable alcohol dehydroge ...
Thermococcus kodakarensis
Appl. Environ. Microbiol.
79
2209-2217
2013
-
-
1
-
-
-
6
9
-
-
3
48
10
1
-
-
1
-
-
-
-
-
48
1
1
1
-
1
9
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
6
-
9
-
-
3
48
10
-
-
1
-
-
-
-
48
1
1
-
1
9
2
-
-
-
-
-
-
-
9
9
740200
Kulig
Biochemical characterization o ...
Ralstonia sp., Ralstonia sp. DSM 6428
Biotechnol. Bioeng.
110
1838-1848
2013
-
-
1
-
-
-
2
9
-
1
-
-
-
4
-
-
1
-
-
-
16
2
53
-
2
-
-
1
-
2
-
1
2
2
-
-
-
-
1
2
-
-
-
-
2
2
9
-
1
-
-
-
-
-
1
-
-
16
2
53
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
740851
Ma
Characterization and cofactor ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM2026
J. Microbiol. Biotechnol.
23
1699-1707
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
12
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
-
-
1
-
-
-
-
3
-
1
1
-
3
4
-
-
1
-
-
-
-
1
8
1
1
1
1
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
3
-
-
1
-
-
-
1
8
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
-
-
1
-
-
1
-
5
-
1
2
-
4
2
-
-
1
-
-
-
-
2
14
1
2
1
1
-
-
2
-
1
2
-
-
-
-
-
1
2
-
-
1
-
-
-
5
-
1
2
-
4
-
-
1
-
-
-
2
14
1
1
1
-
-
2
-
1
-
-
-
-
-
5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Hungateiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
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4
-
2
1
-
-
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-
-
-
-
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1
-
1
-
-
-
-
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1
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-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
-
-
1
-
-
-
14
5
-
3
3
-
-
7
-
-
1
-
-
-
-
-
30
1
1
1
-
4
5
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
14
-
5
-
3
3
-
-
-
-
1
-
-
-
-
30
1
1
-
4
5
2
-
-
-
-
-
-
-
5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
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-
-
3
-
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2
-
2
1
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-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
2
-
1
-
-
-
-
5
-
-
-
1
-
1
-
-
1
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
-
-
1
-
-
-
-
6
2
-
1
-
-
1
-
-
-
1
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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6
2
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1
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
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1
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1
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1
2
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
-
-
-
2
-
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-
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2
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2
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2
2
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-
-
-
-
-
-
-
-
287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
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287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
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287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
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287280
Hatanaka
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Purification and properties of ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides IFO 3426
Agric. Biol. Chem.
38
1819-1833
1974
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14
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287281
Rhodes
-
Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
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287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
J. Biol. Chem.
243
4372-4379
1968
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