Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Gluconobacter oxydans | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 40% loss of activity | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.073 | - |
NADP+ | - |
Gluconobacter oxydans | |
20.6 | - |
D-gluconate | - |
Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Gluconobacter oxydans | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM, increases the activity to 127% | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
subsp. oxydans DSM3503 | - |
Purification (Comment) | Organism |
---|---|
- |
Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-dehydro-D-gluconate + NADPH | - |
Gluconobacter oxydans | D-gluconate + NADP+ | - |
r | |
D-fructose + NADPH | 10% of the activity with 5-dehydrydro-D-gluconate | Gluconobacter oxydans | 5-dehydro-D-fructose + NADP+ | - |
? | |
D-gluconate + NADP+ | - |
Gluconobacter oxydans | 5-dehydro-D-gluconate + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000, SDS-PAGE | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
reduction of 5-dehydro-D-gluconate | Gluconobacter oxydans |
10 | - |
- |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | cofactor | Gluconobacter oxydans |