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Literature summary for 1.1.1.62 extracted from
- 17beta-Estradiol dehydrogenase of ovine ovaries (1970), J. Biol. Chem., 245, 1978-1984.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 104000 | - |
sucose density gradient centrifugation, gel filtration | Ovis aries |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ovary | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.136 | - |
- |
Ovis aries |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C, most stable between pH 6.5 and 7.0 | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-O-methyl-17beta-estradiol + NAD+ | 85% of the activity with estradiol-17beta | Ovis aries | 3-O-methylestrone + NADH | - |
? |  |
| estradiol-17beta + NAD+ | reversible at neutral pH, reduction of estrone at 5% of the activity of reduction of estradiol-17beta | Ovis aries | estrone + NADH | - |
r | |
| estradiol-17beta + NADP+ | reversible at neutral pH, reduction of estrone at 5% of the activity of reduction of estradiol-17beta | Ovis aries | estrone + NADPH | - |
r | |
| estradiol-17beta + NADP+ | 17% of the activity with NAD+ | Ovis aries | estrone + NADPH | - |
r | |
| estrone + NADH | reversible at neutral pH, reduction of estrone at 5% of the activity of reduction of estradiol-17beta | Ovis aries | estradiol-17beta + NAD+ | - |
r | |
| estrone + NADPH + H+ | reversible at neutral pH, reduction of estrone at 5% of the activity of oxidation of estradiol-17beta | Ovis aries | estradiol-17beta + NADP+ | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
NADP+, NADPH, MnSO4 and (NH4)2SO4 are detrimental to stability | Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
estradiol-17beta + NAD+ | Ovis aries |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 9.2 | pH 6.7: about 45% of maximal activity, pH 9.2: about 60% of maximal activity | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | cofactor | Ovis aries | |
| NADH | cofactor | Ovis aries | |
| NADP+ | cofactor | Ovis aries | |
| NADP+ | 17% of the activity with NAD+ | Ovis aries | |
| NADPH | cofactor | Ovis aries | |
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Release 2023.1 (January 2023)
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