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Literature summary for 1.1.1.62 extracted from

  • Renwick, A.G.C.; Soon, C.Y.; Chambers, S.M.; Brown, C.R.
    Estradiol-17beta dehydrogenase from chicken liver (1981), J. Biol. Chem., 256, 1881-1887.
    View publication on PubMed
Search for more literature for 1.1.1.62

Inhibitors

Inhibitors Comment Organism Structure
16alpha-Bromoacetoxyprogesterone
-
 Gallus gallus
PCMB 0.1 mM, complete loss of activity Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
 NADP+
-
 Gallus gallus
0.0173
-
 estradiol-17beta in absence of bovine serum albumin Gallus gallus
0.025
-
 estradiol-17beta in presence of bovine serum albumin Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 and a second enzyme form of 97000 Da, gel filtration Gallus gallus
97000
-
 and a second enzyme form of 97000 Da Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
289
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
-20°C, 50% v/v glycerol, stable for at least 3 months Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epitestosterone + NADP+ poor substrate Gallus gallus 4-androsten-3,17-dione + NADPH + H+
-
 ?
estradiol-17alpha + NADP+ at about 3% of the activity with estradiol-17beta Gallus gallus estrone + NADPH
-
 ?
estradiol-17beta + NADP+ rate of oxidation of estradiol-17beta at the optimum, pH 9.9, is three to four times faster than that of the reverse reaction at pH 7.4 Gallus gallus estrone + NADPH
-
 r
estrone + NADPH + H+ rate of oxidation of estradiol-17beta at the optimum, pH 9.9, is three to four times faster than that of the reverse reaction at pH 7.4 Gallus gallus estradiol-17beta + NADP+
-
 r
testosterone + NADP+ poor substrate Gallus gallus 4-androstene-3,17-dione + NADPH + H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.9
-
 NADP+-linked oxidation of estradiol-17beta Gallus gallus

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Gallus gallus
NADPH cofactor Gallus gallus