Inhibitors | Comment | Organism | Structure |
---|---|---|---|
16alpha-Bromoacetoxyprogesterone | - |
Gallus gallus | |
PCMB | 0.1 mM, complete loss of activity | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
NADP+ | - |
Gallus gallus | |
0.0173 | - |
estradiol-17beta | in absence of bovine serum albumin | Gallus gallus | |
0.025 | - |
estradiol-17beta | in presence of bovine serum albumin | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
and a second enzyme form of 97000 Da, gel filtration | Gallus gallus |
97000 | - |
and a second enzyme form of 97000 Da | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
289 | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-20°C, 50% v/v glycerol, stable for at least 3 months | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epitestosterone + NADP+ | poor substrate | Gallus gallus | 4-androsten-3,17-dione + NADPH + H+ | - |
? | |
estradiol-17alpha + NADP+ | at about 3% of the activity with estradiol-17beta | Gallus gallus | estrone + NADPH | - |
? | |
estradiol-17beta + NADP+ | rate of oxidation of estradiol-17beta at the optimum, pH 9.9, is three to four times faster than that of the reverse reaction at pH 7.4 | Gallus gallus | estrone + NADPH | - |
r | |
estrone + NADPH + H+ | rate of oxidation of estradiol-17beta at the optimum, pH 9.9, is three to four times faster than that of the reverse reaction at pH 7.4 | Gallus gallus | estradiol-17beta + NADP+ | - |
r | |
testosterone + NADP+ | poor substrate | Gallus gallus | 4-androstene-3,17-dione + NADPH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.9 | - |
NADP+-linked oxidation of estradiol-17beta | Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cofactor | Gallus gallus | |
NADPH | cofactor | Gallus gallus |