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Literature summary for 1.1.1.60 extracted from
- Tartronic semialdehyde reductase (crystalline) (1966), Methods Enzymol., 9, 240-247.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fluoroacetate | - |
Pseudomonas putida |  |
| glycolate | - |
Pseudomonas putida | |
| glyoxylate | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | 0.027 | NADH | - |
Pseudomonas putida | |
| 0.036 | 0.05 | NADPH | - |
Pseudomonas putida | |
| 0.05 | 0.4 | D-glycerate | - |
Pseudomonas putida | |
| 0.05 | 0.4 | tartronate semialdehyde | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 91000 | - |
sedimentation equilibrium | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tartronate semialdehyde + NAD(P)H | Pseudomonas putida | - |
(R)-glycerate + NAD(P)+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160 | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 2°C, suspension in ammonium sulfate, pH 7.5, 10% loss in 1 month | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxypyruvate + NAD(P)H + H+ | - |
Pseudomonas putida | glycerate + NAD(P)+ | - |
r | |
| malonic semialdehyde + NADH | - |
Pseudomonas putida | 3-hydroxypropanoate + NAD+ | - |
? | |
| mesoxalic semialdehyde + NADH | - |
Pseudomonas putida | hydroxypyruvate + NAD+ | - |
? | |
| tartronate semialdehyde + NAD(P)H | - |
Pseudomonas putida | (R)-glycerate + NAD(P)+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 243 | - |
NADH | - |
Pseudomonas putida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.7 | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas putida | |
| NADH | - |
Pseudomonas putida | |
| NADP+ | - |
Pseudomonas putida | |
| NADPH | - |
Pseudomonas putida | |
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Release 2023.1 (January 2023)
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