Cloned (Comment) | Organism |
---|---|
cloning from contaminating bacteria, DNA and amino acid sequence determmination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli train BL21(DE3)pLysS | Serratia sp. |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion, mixing of 0.001 ml 6 mg/ml protein in 150 mM ammonium acetate, 50 mM sodium chloride, 20 mM Tris, pH 7.4, 5% glycerol with 0.001 ml reservoir solution containing 5-10% PEG 3350, 0.2 M calcium acetate, and 4% glycerol, X-ray diffraction structure determination and analysis at 1.90 A resolution, molecular replacement using PDB entry 1jpu as a search model | Serratia sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | active-site zinc responsible for coordinating glycerol in the active site of the enzyme. The active site is highly conserved and contains a zinc ion coordinated by two histidines and an aspartate | Serratia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
- |
Serratia sp. |
160000 | - |
recombinant His6-tagged enzyme, ge filtration | Serratia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Serratia sp. | - |
glycerone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli train BL21(DE3)pLysS by nicke affinity chromatography and gel filtration | Serratia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | - |
Serratia sp. | glycerone + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | protein structure-function relationships | Serratia sp. |
tetramer | 4 * 37000, recombinant His6-tagged enzyme, SDS-PAGE | Serratia sp. |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Serratia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme binding structure, overview | Serratia sp. | |
NADH | - |
Serratia sp. |
General Information | Comment | Organism |
---|---|---|
additional information | protein structure-function relationships | Serratia sp. |
physiological function | glycerol dehydrogenase is the enzyme responsible for the oxidation of glycerol to dihydroxyacetone. This permits its entrance into the glycolytic pathway | Serratia sp. |