Activating Compound | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | at 1 mM 140% of the activity without activator, at 10 mM 220% of the activity without activator | Schizosaccharomyces pombe | |
N-ethylmaleimide | at 0.1 mM 145% of the activity without activator | Schizosaccharomyces pombe | |
NH4+ | - |
Schizosaccharomyces pombe | |
p-chloromercuribenzoic acid | at 0.1 mM 133% of the activity without activator | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Schizosaccharomyces pombe | |
dithiothreitol | - |
Schizosaccharomyces pombe | |
EDTA | 10 mM | Schizosaccharomyces pombe | |
L-cysteine | - |
Schizosaccharomyces pombe | |
Li+ | competitive, forward reaction | Schizosaccharomyces pombe | |
Na+ | competitive, forward reaction | Schizosaccharomyces pombe | |
NADP+ | inhibits oxidation of glycerol | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
dihydroxyacetone | pH 6.0 | Schizosaccharomyces pombe | |
0.12 | - |
NADH | pH 6.0 | Schizosaccharomyces pombe | |
0.13 | - |
NAD+ | pH 10.0 | Schizosaccharomyces pombe | |
0.5 | - |
glycerol | pH 10.0 | Schizosaccharomyces pombe |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Schizosaccharomyces pombe | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Schizosaccharomyces pombe | |
Li+ | - |
Schizosaccharomyces pombe | |
Mn2+ | increases activity significantly | Schizosaccharomyces pombe | |
Zn2+ | increases activity | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
8 * 47000, SDS-PAGE | Schizosaccharomyces pombe |
400000 | - |
gel filtration | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Schizosaccharomyces pombe | - |
dihydroxyacetone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Schizosaccharomyces pombe |
Storage Stability | Organism |
---|---|
-70°C, long term storage | Schizosaccharomyces pombe |
4°C, in 2 M (NH4)2SO4, 1 M glycerol, several months | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2,3-butanetriol + NAD+ | - |
Schizosaccharomyces pombe | 1,3-dihydroxybutane-2-one + NADH | - |
? | |
1,2-butanediol + NAD+ | - |
Schizosaccharomyces pombe | 1-hydroxybutane-2-one + NADH | - |
r | |
1,2-propanediol + NAD+ | - |
Schizosaccharomyces pombe | hydroxyacetone + NADH | - |
r | |
2,3-butanediol + NAD+ | - |
Schizosaccharomyces pombe | 3-hydroxybutane-2-one + NADH | - |
r | |
DL-glyceraldehyde + NAD+ | - |
Schizosaccharomyces pombe | 3-hydroxypyruvaldehyde + NADH | - |
? | |
ethanediol + NAD+ | - |
Schizosaccharomyces pombe | glycolaldehyde + NADH | - |
? | |
glycerol + NAD+ | - |
Schizosaccharomyces pombe | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Schizosaccharomyces pombe | dihydroxyacetone + NADH + H+ | - |
r | |
methylglyoxal + NADH | - |
Schizosaccharomyces pombe | lactaldehyde + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 47000, SDS-PAGE | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | reduction of dihydroxyacetone | Schizosaccharomyces pombe |
11 | 12 | oxidation of glycerol | Schizosaccharomyces pombe |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | pH 5.5: about 70% of maximal activity, pH 9.0: about 45% of maximal activity, reduction of dihydroxyacetone | Schizosaccharomyces pombe |
9 | 12 | pH 9.0: about 45% of maximal activity, pH 11-12: maximal activity, oxidation of glycerol | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Schizosaccharomyces pombe | |
NADH | - |
Schizosaccharomyces pombe |