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Literature summary for 1.1.1.58 extracted from
- D-Altronate: NAD-oxidoreductase in Escherichia coli K12. Purification, properties, and specificity (1972), Eur. J. Biochem., 26, 50-61.
General Stability
| General Stability | Organism |
|---|---|
| altronate protects against heat denaturation | Escherichia coli |
| fructuronate does not protect against heat denaturation | Escherichia coli |
| mannonate protects against heat denaturation | Escherichia coli |
| NAD+ does not protect against heat denaturation | Escherichia coli |
| NADH protects against heat denaturation | Escherichia coli |
| NADPH protects against heat denaturation | Escherichia coli |
| tagaturonate does not protect against heat denaturation | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli |  |
| additional information | products inhibition with all products in forward and reverse reaction | Escherichia coli | |
| p-chloromercuribenzoate | non-competitive, Ki: 1 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
NADH | - |
Escherichia coli | |
| 0.11 | - |
NAD+ | - |
Escherichia coli | |
| 0.35 | - |
tagaturonate | - |
Escherichia coli | |
| 0.4 | - |
NADPH | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-altronate + NAD+ | Escherichia coli | - |
D-tagaturonate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Escherichia coli |
| 328 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, several months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-altronate + NAD+ | - |
Escherichia coli | D-tagaturonate + NADH | - |
r | |
| D-tagaturonate + NADPH + H+ | - |
Escherichia coli | D-altronate + NADP+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
NADH oxidation | Escherichia coli |
| 8.9 | - |
NAD+ reduction | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | at pH 6 and 8: about 50% of activity maximum | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADP+ | Escherichia coli | |
| NAD+ | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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