General Stability | Organism |
---|---|
sensitive to proteolysis with Glu-C protease and trypsin, which can be nearly completly hindered by presence of NADH or cortisone | Streptomyces exfoliatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition with iodoacetate, acetylsialic acid, phenylmethylsulfonyl fluoride, glyoxal, iodoacetamide, dimethylaminoisopropyl ethylene dicarbodiimide, acetylimidazol, diethyl pyrocarbonate | Streptomyces exfoliatus | |
pyridoxal 5'-phosphate | slightly | Streptomyces exfoliatus | |
Tetranitromethane | other Tyr-selective reagents, Tyr residues are labeled with nitro-group by inhibitor | Streptomyces exfoliatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha,20beta-hydroxysteroid androstanes or pregnane derivatives + NAD(P)H | Streptomyces exfoliatus | enzyme belongs to the group of short-chain dehydrogenases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces exfoliatus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha,20beta-hydroxysteroid androstanes or pregnane derivatives + NAD(P)H | enzyme belongs to the group of short-chain dehydrogenases | Streptomyces exfoliatus | ? | - |
? | |
cortisone + NAD(P)H | - |
Streptomyces exfoliatus | 17alpha,20beta,21-trihydroxy-pregn-4-ene-3,11-dione + NAD(P)+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
low stability | Streptomyces exfoliatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | protects enzyme almost completely against proteolysis with Glu-C protease and trypsin | Streptomyces exfoliatus |