Cloned (Comment) | Organism |
---|---|
His6-tagged 17beta-HSD2 overexpressed in Sf9 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-octylglucoside | strong | Homo sapiens | |
dodecyl-beta-D-maltoside | inhibition above 0.5% | Homo sapiens | |
Triton X-100 | significant | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
dihydrotestosterone | pH 7.4, with NAD+ as cofactor | Homo sapiens | |
0.00035 | - |
estradiol | pH 7.4, with NAD+ as cofactor | Homo sapiens | |
0.00053 | - |
20alpha-dihydroprogesterone | pH 7.4, with NAD+ as cofactor | Homo sapiens | |
0.00061 | - |
testosterone | pH 7.4, with NAD+ as cofactor | Homo sapiens | |
0.0053 | - |
NADH | pH 7.4, reaction with 4-dionetestosterone | Homo sapiens | |
0.0056 | - |
NADH | pH 7.4, reaction with estronel | Homo sapiens | |
0.11 | - |
NAD+ | pH 7.4, reaction with estradiol | Homo sapiens | |
0.167 | - |
NAD+ | pH 7.4, reaction with testosterone | Homo sapiens | |
9.6 | - |
NADP+ | pH 7.4, reaction with estradiol | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42300 | - |
2 * 42300, SDS-PAGE | Homo sapiens |
90400 | - |
glycerol gradient ultracentrifugation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His6-tagged 17beta-HSD2 overexpressed in Sf9 cells | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, activity in both the homogenate and the supernatant using dodecyl-beta-D-maltoside as detergent can be kept for several days without significant loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
20alpha-dihydroprogesterone + NAD+ | - |
Homo sapiens | 4-pregnen-3alpha,20beta-diol + NADH | - |
r | |
20alpha-dihydroprogesterone + NADP+ | - |
Homo sapiens | 4-pregnen-3alpha,20beta-diol + NADPH | - |
r | |
dihydrotestosterone + NAD+ | - |
Homo sapiens | 5alpha-androstan-3,17-dione + NADH | - |
r | |
dihydrotestosterone + NADP+ | - |
Homo sapiens | 5alpha-androstan-3,17-dione + NADPH | - |
r | |
estradiol + NAD+ | - |
Homo sapiens | estrone + NADH | - |
? | |
estradiol + NADP+ | - |
Homo sapiens | estrone + NADPH | - |
? | |
testosterone + NAD+ | - |
Homo sapiens | androst-4-en-3,17-dione + NADH + H+ | - |
r | |
testosterone + NADP+ | - |
Homo sapiens | androst-4-en-3,17-dione + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42300, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
17beta-HSD2 | - |
Homo sapiens |
17beta-hydroxysteroid dehydrogenase 2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
NADH | pH 7.4, reaction with 4-dionetestosterone | Homo sapiens | |
0.33 | - |
NADH | pH 7.4, reaction with estronel | Homo sapiens | |
0.78 | - |
20alpha-dihydroprogesterone | pH 7.4, with NAD+ as cofactor | Homo sapiens | |
1.3 | - |
NADP+ | pH 7.4, reaction with estradiol | Homo sapiens | |
2.4 | - |
dihydrotestosterone | pH 7.4, with NAD+ as cofactor | Homo sapiens | |
2.9 | - |
NAD+ | pH 7.4, reaction with testosterone | Homo sapiens | |
3.4 | - |
testosterone | pH 7.4, with NAD+ as cofactor | Homo sapiens | |
3.9 | - |
estradiol | pH 7.4, with NAD+ as cofactor | Homo sapiens | |
4.7 | - |
NAD+ | pH 7.4, reaction with estradiol | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | prefers NAD+ over NADP+ | Homo sapiens | |
NADH | - |
Homo sapiens | |
NADP+ | prefers NAD+ over NADP+ | Homo sapiens | |
NADPH | - |
Homo sapiens |