Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fluoxetine | activates allopregnanolone formation in vivo, not in vitro at a concentration up to 0.05 mM, therefore in vivo activation is probably not realized by enzyme activation but a different mechanism | Homo sapiens | |
additional information | no in vitro activation by paroxetine, sertraline, norfluoxetine, carbamazepine, clozapine, flurbiprofen, and sulfobromophthalein at a concentration up to 0.05 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
from human cDNA library, expression as GST-fusion protein in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ursodeoxycholate | forward reaction: competitive against 5alpha-dihydroprogesteroneand noncompetitive against NADPH, reverse reaction: competitive against allopregnanolone and noncompetitive against NADP+ | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, ternary complex, sequential, kinetic mechanism of allopregnanolone synthesis with the cofactor binding before the steroid and leaving after it | Homo sapiens | |
0.00007 | - |
NADPH | pH 7.0, 25°C | Homo sapiens | |
0.00023 | - |
NADP+ | pH 7.0, 25°C | Homo sapiens | |
0.00051 | - |
5beta-dihydroprogesterone | pH 7.0, 37°C | Homo sapiens | |
0.0015 | - |
allopregnanolone | pH 7.0, 25°C | Homo sapiens | |
0.0018 | - |
5alpha-dihydroprogesterone | pH 7.0, 25°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydroprogesterone + NADPH + H+ | Homo sapiens | last step in neurosteroid allopregnanolone biosynthesis | 3alpha,5alpha-3-hydroxypregnan-20-one + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
type III enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion protein from Escherichia coli by affinity chromatography on a glutathione resin, cleavage of the GST-tag by thrombin | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ | reaction mechanism | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydroprogesterone + NADPH + H* | - |
Homo sapiens | 3alpha,5alpha-3-hydroxypregnan-20-one + 5alpha,20alpha-tetrahydroprogesterone + NADP+ | - |
? | |
5alpha-dihydroprogesterone + NADPH + H+ | last step in neurosteroid allopregnanolone biosynthesis | Homo sapiens | 3alpha,5alpha-3-hydroxypregnan-20-one + NADP+ | - |
? | |
5beta-dihydroprogesterone + NADPH + H+ | preferred substrate | Homo sapiens | 3alpha,5beta-3-hydroxypregnan-20-one + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD | - |
Homo sapiens |
3alpha-hydroxysteroid dehydrogenase | - |
Homo sapiens |
AKR1C2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, both reaction directions | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
NADP+ | pH 7.0, 25°C | Homo sapiens | |
0.00017 | - |
allopregnanolone | pH 7.0, 25°C | Homo sapiens | |
0.003 | - |
NADPH | pH 7.0, 25°C | Homo sapiens | |
0.003 | - |
5alpha-dihydroprogesterone | pH 7.0, 25°C | Homo sapiens | |
0.0167 | - |
5beta-dihydroprogesterone | pH 7.0, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, both reaction directions | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Homo sapiens | |
0.000027 | - |
ursodeoxycholate | pH 7.0, 25°C, versus allopregnanolone | Homo sapiens | |
0.000086 | - |
ursodeoxycholate | pH 7.0, 25°C, versus NADP+ | Homo sapiens | |
0.00021 | - |
ursodeoxycholate | pH 7.0, 25°C, versus 5alpha-dihydroprogesterone | Homo sapiens | |
0.00055 | - |
ursodeoxycholate | pH 7.0, 25°C, versus NADPH | Homo sapiens |