Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
General Stability | Organism |
---|---|
mercaptoethanol stabilizes | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heavy metal ions | - |
Pseudomonas putida | |
p-chloromercuribenzoate | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | 0.025 | cholic acid | - |
Pseudomonas putida | |
0.87 | - |
NAD+ | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
sedimentation equilibrium centrifugation | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Storage Stability | Organism |
---|---|
0-3°C, lyophilized, at least 6 months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha-hydroxy-12-oxo-9,11-cholenic acid + NADP+ | - |
Pseudomonas putida | 3,12-dioxo-9,11-cholenic acid + NADPH | - |
? | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | i.e. 5alpha-androstan-17beta-ol-3-one | Pseudomonas putida | 5alpha-androstan-3alpha,17beta-diol + NAD(P)+ | - |
r | |
5alpha-dihydrotestosterone + NAD(P)H + H+ | i.e. 5alpha-androstan-17beta-ol-3-one | Pseudomonas putida | (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+ | - |
r | |
androsterone + NAD(P)+ | i.e. 3alpha-hydroxy-5alpha-androstan-17-one | Pseudomonas putida | 5alpha-androstan-3,17-dione + NAD(P)H | - |
r | |
chenodeoxycholic acid + NAD(P)+ | i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid | Pseudomonas putida | 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
r | |
cholic acid + NAD(P)+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid | Pseudomonas putida | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
deoxycholic acid + NAD(P)+ | i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid | Pseudomonas putida | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ | - |
? | |
etiocholan-3alpha-ol-17-one + NADP+ | - |
Pseudomonas putida | etiocholan-3,17-dione + NADPH | - |
? | |
lithocholic acid + NADP+ | i.e. 3alpha-hydroxy-5beta-cholan-24-oic acid | Pseudomonas putida | 3-oxo-5beta-cholan-24-oic acid + NADPH | - |
? | |
additional information | acts faster on A/B cis hydroxysteroids than on A/B trans hydroxysteroids | Pseudomonas putida | ? | - |
? | |
additional information | absolutely specific for 3alpha-hydroxy group of C19, C21 and C24 steroids | Pseudomonas putida | ? | - |
? | |
taurocholic acid + NAD(P)+ | i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine | Pseudomonas putida | 2[[7alpha,12alpha-dihydroxy-3,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, sedimentation equilibrium, presence of 2-mercaptoethanol | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min stable in presence of mercaptoethanol, 10% loss of activity in absence of mercaptoethanol | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
reduction of etiocholan-3,17-dione | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | stable for 30 min at 30°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | see also EC 1.1.1.213 for enzymes catalyzing the same reaction but with A-specificity concerning the cofactor, all enzymes without information of stereospecificity for cofactors are included in EC 1.1.1.50, but some of those may in fact belong to EC 1.1.1.213 | Pseudomonas putida |