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Literature summary for 1.1.1.49 extracted from
- Overexpression, purification and enzymatic characterization of a recombinant plastidial glucose-6-phosphate dehydrogenase from barley (Hordeum vulgare cv. Nure) roots (2013), Plant Physiol. Biochem., 73, 266-273.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Hordeum vulgare |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0066 | - |
NADP+ | native enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
| 0.015 | - |
NADP+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
| 0.78 | - |
D-glucose 6-phosphate | native enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
| 0.86 | - |
D-glucose 6-phosphate | recombinant His-tagged enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Hordeum vulgare | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55800 | - |
- |
Hordeum vulgare |
| 57000 | - |
- |
Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | Hordeum vulgare | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| dithiothreitol | the recombinant His-tagged enzyme shows 60% residual activity after 1 h at 25 mM dithiothreitol | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
cultivar Nure | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Blue-Sepharose column chromatography, Resource-Q column chromatography, and HiTrap column chromatography | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Hordeum vulgare | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | - |
Hordeum vulgare | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55800, calculated from amino acid sequence | Hordeum vulgare |
| ? | x * 57000, native enzyme, SDS-PAGE | Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P2-G6PDH | - |
Hordeum vulgare |
| plastidial glucose 6-phosphate dehydrogenase | - |
Hordeum vulgare |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Hordeum vulgare | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
NADPH | recombinant His-tagged enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
| 0.095 | - |
NADPH | native enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
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