Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | after pathogen infection with Phytophthora nicotianae, G6PDH activity rises 2 to 3fold in leaves of a resistant, but not of a susceptible, tobacco cultivar | Nicotiana tabacum |
Application | Comment | Organism |
---|---|---|
additional information | because isoenzyme replacement of G6PDH in the cytosol of tobacco is beneficial under various kinds of cues, this strategy may be a tool to enhance stress tolerance in general | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
into vector pET16b and expressed in a G6PDH-deficient Escherichia coli strain. Overexpression in the cytosol of the susceptible tobacco cultivar Xanthi | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glucosamine 6-phosphate | competitive G6PDH inhibitor | Nicotiana tabacum | |
NADPH | - |
Arabidopsis thaliana | |
NADPH | - |
Nicotiana tabacum | |
RNAi | suppression of endogenous cytosolic G6PDH isoforms result in highly uniform defense responses and also enhanced drought tolerance and flowering | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NADP+ | - |
Arabidopsis thaliana | |
0.041 | - |
NADP+ | G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars | Nicotiana tabacum | |
0.041 | - |
NADP+ | G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars | Nicotiana tabacum | |
0.24 | - |
D-glucose 6-phosphate | G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars | Nicotiana tabacum | |
0.25 | - |
D-glucose 6-phosphate | G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars | Nicotiana tabacum | |
0.57 | - |
D-glucose 6-phosphate | - |
Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic G6PDH seems to be a crucial factor for the outcome of plant defense responses | Nicotiana tabacum | 5829 | - |
plastid | - |
Arabidopsis thaliana | 9536 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8L743 | - |
- |
Nicotiana tabacum | - |
var. Samsun, resistant SNN cultivar and susceptible Xanthi cultivar | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Nicotiana tabacum | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Arabidopsis thaliana | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
additional information | transgenic (previously susceptible) lines overexpressing G6PDH-encoding gene, displaying high NADPH tolerance engineered for cytosolic expression, show early oxidative bursts, callose deposition, and changes in metabolic parameter | Nicotiana tabacum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PDH | - |
Nicotiana tabacum |
G6PDH | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Nicotiana tabacum | |
NADP+ | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADPH | G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars | Nicotiana tabacum | |
0.051 | - |
NADPH | G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars | Nicotiana tabacum | |
0.06 | - |
NADPH | - |
Arabidopsis thaliana |