Application | Comment | Organism |
---|---|---|
drug development | Trypanosoma brucei G6PDH is a valid drug target for human steroids. Inhibition of G6PDH by dehydroepiandrosterone derivatives may lead to the development of a new class of anti-trypanosomatid compounds | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
N-terminally His-tagged recombinant G6PDH expressed in Escherichia coli BL21 transformed with recombinant pET28 plasmid | Trypanosoma brucei |
N-terminally His-tagged recombinant G6PDH expressed in Escherichia coli BL21 transformed with recombinant pET28 plasmid | Leishmania mexicana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dehydroepiandrosterone | uncompetitively inhibits bloodstream form cells | Trypanosoma brucei | |
epiandrosterone | uncompetitively inhibits bloodstream form cells | Trypanosoma brucei | |
additional information | is not inhibited by dehydroepiandrosterone and epiandrosterone | Leishmania mexicana | |
RNAi | mediates reduction of the G6PDH level in bloodstream form cells | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
NADP+ | - |
Trypanosoma brucei | |
0.0121 | - |
NADP+ | - |
Leishmania mexicana | |
0.0578 | - |
D-glucose 6-phosphate | - |
Trypanosoma brucei | |
0.0745 | - |
D-glucose 6-phosphate | - |
Leishmania mexicana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | - |
- |
- |
Trypanosoma brucei | - |
427 strain | - |
Trypanosoma brucei 427 | - |
427 strain | - |
Purification (Comment) | Organism |
---|---|
to homogeneity by a single nickel affinity chromatographic step | Trypanosoma brucei |
to homogeneity by a single nickel affinity chromatographic step | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Trypanosoma brucei | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Leishmania mexicana | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Trypanosoma brucei 427 | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PDH | - |
Trypanosoma brucei |
G6PDH | - |
Leishmania mexicana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.4 | - |
D-glucose 6-phosphate | - |
Trypanosoma brucei | |
22.2 | - |
D-glucose 6-phosphate | - |
Leishmania mexicana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00046 | - |
epiandrosterone | in the presence of NADP+ | Trypanosoma brucei | |
0.00049 | - |
epiandrosterone | in the presence of D-glucose 6-phosphate | Trypanosoma brucei | |
0.0011 | - |
dehydroepiandrosterone | in the presence of NADP+ | Trypanosoma brucei | |
0.0017 | - |
dehydroepiandrosterone | in the presence of D-glucose 6-phosphate | Trypanosoma brucei |