Literature summary for 1.1.1.49 extracted from

Oezmen, I.; Ciftci, M.; Kufrevioglu, O.I.; Curuk, M.A.
Investigation of glucose 6-phosphate dehydrogenase (G6PD) kinetics for normal and G6PD-deficient persons and the effects of some drugs (2004), J. Enzyme Inhib. Med. Chem., 19, 45-50.

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Activating Compound

Activating Compound	Comment	Organism	Structure
metamizol	activation of mutant enzymes 1 and 2	Homo sapiens
streptomycin	activation of mutant enzymes 1 and 2	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
metamizol	inhibition of wild-type and mutant enzyme 3	Homo sapiens
netilmicin	inhibition of wild-type and mutant enzymes 1-3	Homo sapiens
sodium ceftizoxime	inhibition of wild-type and mutant enzymes 1-3	Homo sapiens
sodium cefuroxime	inhibition of wild-type and mutant enzymes 1-3	Homo sapiens
streptomycin	inhibition of wild-type and mutant enzyme 3	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.048	-	D-glucose 6-phosphate	pH 8.0, 25, mutant 2	Homo sapiens
0.07	-	NADP+	pH 8.0, 25, mutant enzyme 2	Homo sapiens
0.083	-	D-glucose 6-phosphate	pH 8.0, 25, mutant 3	Homo sapiens
0.085	-	NADP+	pH 8.0, 25, mutant enzyme 3	Homo sapiens
0.13	-	NADP+	pH 8.0, 25, mutant enzyme 1	Homo sapiens
0.15	-	D-glucose 6-phosphate	pH 8.0, 25, mutant 1	Homo sapiens
0.21	-	NADP+	pH 8.0, 25, wild-type enzyme	Homo sapiens
0.5	-	D-glucose 6-phosphate	pH 8.0, 25, wild-type enzyme	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	Homo sapiens	key enzyme catalyzing the first step in the pentose phosphate pathway	D-glucono-1,5-lactone 6-phosphate + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	wild-type and 3 enzyme-deficient children, i.e. mutants 1,2, and 3	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type enzyme, 4.1fold from blood by ammonium sulfate precipitation, dialysis, and 2',5'-ADP affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
blood	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
27.5	-	purified wild-type enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	-	Homo sapiens	D-glucono-1,5-lactone 6-phosphate + NADPH + H+	-	?
D-glucose 6-phosphate + NADP+	key enzyme catalyzing the first step in the pentose phosphate pathway	Homo sapiens	D-glucono-1,5-lactone 6-phosphate + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
?	59000-60000, wild-type and mutant enzymes, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
G6PD	-	Homo sapiens
glucose-6-phosphate dehydrogenase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	wild-type enzyme	Homo sapiens
40	-	mutant enzymes 1 and 3	Homo sapiens
45	-	mutant enzyme 2	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	mutant enzymes are more thermostable than the wild-type enzyme	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	wild-type enzyme	Homo sapiens
8.5	-	mutant enzymes 1 and 3	Homo sapiens
9	-	mutant enzyme 2	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	-	Homo sapiens

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Release 2023.1 (January 2023)