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Literature summary for 1.1.1.49 extracted from
- Purification and characterization of glucose 6-phosphate dehydrogenase from sheep erythrocytes and inhibitory effects of some antibiotics on enzyme activity (2002), J. Enzyme Inhib. Med. Chem., 17, 271-277.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| amikacin | in vitro, noncompetitive | Ovis aries |  |
| gentamicin sulfate | in vitro, noncompetitive | Ovis aries | |
| penicillin G potassium | in vitro, noncompetitive | Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000012 | - |
NADP+ | pH 8.0, 25°C | Ovis aries | |
| 0.036 | - |
D-glucose 6-phosphate | pH 8.0, 25°C | Ovis aries | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66880 | - |
2 * 66880, SDS-PAGE | Ovis aries |
| 120000 | - |
gel filtration | Ovis aries |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | Ovis aries | key enzyme catalyzing the first step of the hexose monophosphate shunt pathway | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1189fold by ammonium sulfate precipitation and 2',5'-ADP affinity chromatography | Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.64 | - |
purified enzyme | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | - |
Ovis aries | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
| D-glucose 6-phosphate + NADP+ | key enzyme catalyzing the first step of the hexose monophosphate shunt pathway | Ovis aries | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 66880, SDS-PAGE | Ovis aries |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G6PD | - |
Ovis aries |
| glucose-6-phosphate dehydrogenase | - |
Ovis aries |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ovis aries |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
stable | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Ovis aries | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.308 | - |
gentamicin sulfate | pH 8.0, 25°C | Ovis aries | |
| 0.748 | - |
penicillin G potassium | pH 8.0, 25°C | Ovis aries | |
| 12.25 | - |
(((6-(4-tolyl)thieno[2,3-d]pyrimidin-4-yl)amino)methylene)diphosphonic acid | pH 8.0, 25°C | Ovis aries | |
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