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Literature summary for 1.1.1.49 extracted from

  • Grabowska, D.; Jablonska-Skwiecinska, E.; Plochocka, D.; Chelstowska, A.; Lewandowska, I.; Witos, I.; Majewska, Z.; Rokicka-Milewska, R.; Burzynska, B.
    A novel mutation in the glucose-6-phosphate dehydrogenase gene in a subject with chronic nonspherocytic hemolytic anemia--characterization of enzyme using yeast expression system and molecular modeling (2004), Blood Cells Mol. Dis., 32, 124-130.
    View publication on PubMed
Search for more literature for 1.1.1.49

Cloned(Commentary)

Cloned (Comment) Organism
gene g6pd is localized in the Xq28 region, DNA sequence determination and analysis and expression of wild-type and mutant P409R in Saccharomyces cerevisiae, functional complementation of the yeast enzyme deficient mutant strain ZWF1 by the wild-type and mutant P409R Homo sapiens

Protein Variants

Protein Variants Comment Organism
P409R natural occurring point mutation, reconstructed by site-directed mutagenesis, the gene g6pd is highly polymorphic with over 130 mutations identified, reduced activity drastically altered kinetics, and altered tertiary structure, disturbing the binding of NADP+, compared to the wild-type enzyme, reduced thermal stbility Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
 D-glucose 6-phosphate pH 8.0, 37°C, native wild-type enzyme Homo sapiens
0.083
-
 D-glucose 6-phosphate pH 8.0, 37°C, recombinant wild-type enzyme Homo sapiens
0.286
-
 D-glucose 6-phosphate pH 8.0, 37°C, recombinant mutant P409R Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NADP+ Homo sapiens catalyzes the first step of the pentose phosphate pathway, enzyme deficiency often causes hemolytic anaemia D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 wild-type and deficient mutant
-

Purification (Commentary)

Purification (Comment) Organism
native wild-type from blood leukocyte, and recombinant wild-type and mutant P409R from Saccharomyces cerevisiae, by 2',5'-ADP affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Homo sapiens
-
leukocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose 6-phosphate + NADP+ wild-type and mutant P409R, below 10% activity compared to D-glucose 6-phosphate Homo sapiens 2-deoxy-D-glucono-1,5-lactone 6-phosphate + NADPH
-
 ?
D-glucose 6-phosphate + NADP+ catalyzes the first step of the pentose phosphate pathway, enzyme deficiency often causes hemolytic anaemia Homo sapiens D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
D-glucose 6-phosphate + NADP+ wild-type and mutant P409R Homo sapiens D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
More molecular modeling of the tertiary structure of wild-type and mutant enzymes Homo sapiens

Synonyms

Synonyms Comment Organism
G6PD
-
 Homo sapiens
glucose-6-phosphate dehydrogenase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 60 min, the wild-type enzyme is completely stable, the mutant P409R shows 86% reduced thermal stability compared to the wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+ binding by mutant P409R is reduced Homo sapiens